Separation, Purification and Structural Analysis of Polysaccharides from Aloe

Abstract

Abstract: Two kinds of acid polysaccharides, PSA1 and PSA2, were extracted by hot water, precipated by ethanol with different concentrations from Aloe barbadensis miller, deproteinized by enzymolysis and Sevag method. Each of them was shown to be a single homogeneous component by paper and Sephadex column chromatography. TLC analysis of the acid hydrolyzed product and GC/MS analysis of the acetylized product show that PSA1 is composed of D-mannose and D-glucose in a molar ratio of about 1.3:1, and PSA2 is composed of D-mannose, which was confirmed by IR and ¹HNMR techniques as the partially acetylized polymannose. Hakomori methylation, Periodic acid oxidation and Smith degradation of PSA1 and PSA2 show that they are all straight chain polysaccharied with more 1→4 linking, fewer 1→6 linking and few branches.

Key words: Aloe barbadensis miller, Polysaccharides, Structure analysis

CLC Number:

O625

TrendMD:

YAN Ji-Chang, CUI Chun-Yue, ZHANG Yi, CHU Ren-He . Separation, Purification and Structural Analysis of Polysaccharides from Aloe[J]. Chem. J. Chinese Universities, 2003, 24(7): 1189.

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Separation, Purification and Structural Analysis of Polysaccharides from Aloe

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