Preparation and Application of Sol-gel-HBsAb Immunosensor

Abstract

Abstract: Anew type of nonlabbled immunosensor for the determination of hepatitis Bsurface antigen (HBsAg) was made by utilizing Sol-gel techniques to encapsulate hepatitis Bsurface antibody (HBsAb) on tetraethylorthosilicate(TEOS) derived Sol-gel films and then to coat the Sol-gel-HBsAb layer onto the surface of platinum disk electrode. The effective diffusion section area of K₃Fe(CN)₆attained to the platinum electrode has been decreased along with the increase of the singularity reaction immunocompound of HBsAb and HBsAg, the respond current of K₃Fe(CN)₆on the surface of electrode was directly proportional to the effective diffusion section area of K₃Fe(CN)₆attained to the platinum electrode and inversely proportional to the concentration of HBsAg, so HBsAg could be determined indirectly by utilizing K₃Fe(CN)₆as the oxidation-reduction probe. Cyclic voltammetry (CV) was employed to investigate the electrochemical character of the immunosensor in 5 mmol/L K₃Fe(CN)₆-0. 1 mol/L KNO₃-PBSsystem. The linearity of HBsAg in the range of 5-320 μg/Lwith the correlation coefficient of 0. 997 was obtained from differential pulse voltammetry (DPV) by using the three systems. The dependences of the current response on incubation time, sensitivity and reproducibility were studied, and the stability of the sensor was also evaluated. The immunosensor was free from 30 μg/L CEA and 100 μg/L AFP. More than 87. 5% of the results of the human serum samples obtained by this methode were in agreement with those obtained by enzyme-linked assay (ELISA). The immunosensor was found to be stable for about 2 weeks when storing in a dry state at 4 ℃.

Key words: Sol-gel, Immunosensor, Voltammetric determination, HBsAg, HBsAb

CLC Number:

TrendMD:

LIANG Ru-Ping, QIU Jian-Ding, ZOU Xiao-Yong, XU Jian-Jun, CAI Pei-Xiang, MO Jin-Yuan. Preparation and Application of Sol-gel-HBsAb Immunosensor[J]. Chem. J. Chinese Universities, 2003, 24(5): 793.

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