Determination of the Gas-Phase Proton Affinity of N-Dipropyloxy-phosphoryl Alkyl-alanine by Electrospray Ionization Mass Spectrometry with the Kinetic Method

Chem. J. Chinese Universities ›› 2003, Vol. 24 ›› Issue (3): 459.

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Determination of the Gas-Phase Proton Affinity of N-Dipropyloxy-phosphoryl Alkyl-alanine by Electrospray Ionization Mass Spectrometry with the Kinetic Method

CHEN Jing, CHEN Yi, JIANG Yang, ZHAO Yu-Fen

The Key Laboratory of Bioorganic Phosphorus, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China

Received:2001-11-26 Online:2003-03-24 Published:2003-03-24

Abstract

Abstract: In this work, the change of gas-phase proton affinity of alkyl-alanine through the phosphorylation reaction was studied by the electrospray ionization mass spectrometry with kinetic method. It was proved that the phosphorylation could increase the proton affinity of small peptide. A proposal that proton affinity increase might be responsible for the sensitivity improvement of small peptide in mass spectrometry has been provided.

Key words: Gas-phase proton affinity, Electrospray ionization mass spectrometry(ESI-MS), Phosphorylation, Alkyl-alanine

CLC Number:

O657

TrendMD:

CHEN Jing, CHEN Yi, JIANG Yang, ZHAO Yu-Fen . Determination of the Gas-Phase Proton Affinity of N-Dipropyloxy-phosphoryl Alkyl-alanine by Electrospray Ionization Mass Spectrometry with the Kinetic Method[J]. Chem. J. Chinese Universities, 2003, 24(3): 459.

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Determination of the Gas-Phase Proton Affinity of N-Dipropyloxy-phosphoryl Alkyl-alanine by Electrospray Ionization Mass Spectrometry with the Kinetic Method

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