Assignment of the Disulfide Bonds of Huwentoxin-Ⅳ by Partial Reduction and Sequence Analysis

Abstract

Abstract: Huwentoxin-Ⅳ, a neurotoxic peptide from the spider Ornithoctonus huwena, contains three disulfide bonds in a chain of 35 residues.Fully the assignment of disulfide bonds was made by partial reduction and sequence analysis.Huwentoxin-Ⅳ was partially reduced with tris(2-carboxyethyl)-phosphine at 40℃ for 10min at pH=3 and the intermediates were isolated by reversed-phase HPLC.Appropriate isomers containing free thiols were alkylated by adding 100μL of 0.5 mol/Liodoa-cetamide, respectively, following by sequencer analysis, which enabled all three bridges Huwentox-in-Ⅳ to be identified as Cys2-Cys17, Cys9-Cys24 and Cys-Cys31.

Key words: Huwentoxin-Ⅳ, Assignment of disulfide bonds, TCEP, Partial reduction, Sequence determination

CLC Number:

TrendMD:

LIU Zhong-Hua, LIANG Song-Ping . Assignment of the Disulfide Bonds of Huwentoxin-Ⅳ by Partial Reduction and Sequence Analysis[J]. Chem. J. Chinese Universities, 2003, 24(10): 1815.

[1]	CHEN Zhui, TAO Yi, WANG Yi, WANG Yi. Rapid Screening of Lipase Inhibitors from Fructus aurantii Using Lipase-immobilized Magnetic Beads Coupled with High-performance Liquid Chromatography-Mass Spectrometry [J]. Chem. J. Chinese Universities, 2012, 33(12): 2692.
[2]	YI Ping-Gui, YANG Xi-Chun, YU Xian-Yong, LIU Zheng-Jun, LIU Jin, WANG Zhao-Xu, LI Xiao-Fang. Proton Transfer of 2-(2-Amino-3-pyridyl)-benzimidazole Under the Inclusion Interaction with Cucurbit[8]uril [J]. Chem. J. Chinese Universities, 2012, 33(12): 2657.
[3]	ZHAO Yan-Qin, CHEN Jian-Xin. Groups Transfer Reaction of Imidazolinium Salts as Tetrahydrofolate Model Compounds [J]. Chem. J. Chinese Universities, 2012, 33(12): 2670.
[4]	WANG Kai, LIN Xiao, WAN Xing, JIA Tao, ZHANG Xiu-Lan, ZHANG Heng, JU Xiu-Lian. Synthesis of Ruthenium-phenanthroline Porphyrins Interacting with DNA [J]. Chem. J. Chinese Universities, 2012, 33(12): 2663.
[5]	XIAO Tao, ZHOU Xuan, SONG Hao. Stereoselective Construction of the Key Skeleton of Oxindole Alkaloid Humantenine [J]. Chem. J. Chinese Universities, 2012, 33(12): 2676.
[6]	LIU Yu-Chen, LIU Gang, ZHANG Xian-Min, CHEN Jian-Chao, QIU Ming-Hua, QIU De-Wen. New Bibenzyl Derivative from Miao Medicine Herminium bulleyi [J]. Chem. J. Chinese Universities, 2012, 33(12): 2688.
[7]	YU Lan-Lan, RAN Yu, BAI Xi-Xi, LI Ai-Rong, ZHU Yan-Yan, QIN Yun, QU Ling-Bo. Design and Bioactivity of Novel Antimicrobial Peptides and Its Computer Simulation with Phospholipid [J]. Chem. J. Chinese Universities, 2012, 33(12): 2681.
[8]	ZHANG Yan, ZHANG Hua, LIU Ling-Yan, WANG Xin, CHANG Wei-Xing, LI Jing. Barbier-type Propargylation of Aldehyde in Aqueous Media Promoted by Metal Tin [J]. Chem. J. Chinese Universities, 2012, 33(11): 2447.
[9]	KOU Ying-Ying, MENG Qing-Bin, LIU Ke-Liang. Coassembly of Palmitic Acid Modified α-Helical Peptide Amphiphiles [J]. Chem. J. Chinese Universities, 2012, 33(11): 2476.
[10]	ZHANG Zhao, WANG Qi, WU Qiong, HU Xiao-Ying, WANG Cheng-Xi, MEI Wen-Jie, TAO Yun-Yi, WU Wei-Li, ZHENG Wen-Jie. Microwave-assisted Synthesis of Imidazole[4,5f][1,10]phenanthroline Derivatives and Microwave Nonthermal Effect [J]. Chem. J. Chinese Universities, 2012, 33(11): 2441.
[11]	WEN Xia-Xia, NAN Shi-Bin, REN Tian-Rui, YAO Jian-Hua. Interaction Between GABA and Mutant GABAaR [J]. Chem. J. Chinese Universities, 2012, 33(11): 2462.
[12]	WANG Wei, HE Song, LU Yan, ZENG Xian-Shun. Synthesis and Performances of a New Ag⁺-selective Fluorescent Sensor Based on Calix[4]arene [J]. Chem. J. Chinese Universities, 2012, 33(11): 2470.
[13]	LI Xiao, QU Yong-Gang, JIA Peng, LIU Li-Ming, JI Hui-Fan, HE Dong-Yun, JIN Ning-Yi, CHI Bao-Rong. Expression and Characterization of a Recombinant Truncated Capsid Protein of Hepatitis E Virus [J]. Chem. J. Chinese Universities, 2012, 33(11): 2481.
[14]	WEI Tai-Bao, LI Jun-Jian, LIN Qi, YAO Hong, GUO Ying, BAI Cui-Bing, XIE Yong-Qiang, ZHANG You-Ming. Highly Selective Colorimetric Recognition of Copper Ions Based on N-Aryl Coumarin Methyl Ketone Thiosemicarbazone Receptors [J]. Chem. J. Chinese Universities, 2012, 33(11): 2452.
[15]	WANG Lei, FU Ling, JING Lin-Lin, A You-Mei, JIA Lu. Studies of Chemical Constituents from Rosa chinensis Jacq. [J]. Chem. J. Chinese Universities, 2012, 33(11): 2457.

Assignment of the Disulfide Bonds of Huwentoxin-Ⅳ by Partial Reduction and Sequence Analysis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments