Effect of Val162 Mutation on Structure and Function of Cytochrome c Oxidase Soluble Domain

Abstract

Abstract: The effect of Val162 mutation(potentially relative to cancer) on cytochrome coxidase soluble Cu Adomain was studied by site-directed mutagenesis technique combined with UV-Vis, CD, fluorescence spectra, EPR and rapid kinetic methods.It is found that the mutation at the Val162site leads to a structural transformation and decreasing electron transfer activity.It provides important information for understanding the pathological mechanism.

Key words: Cytochrome coxidase, Site-directed mutation and diseases, Structure transformation, Electron transfer

CLC Number:

O614

TrendMD:

SONG Ai-Xin, LI Lian-Zhi, HUANG Zhong-Xian . Effect of Val162 Mutation on Structure and Function of Cytochrome c Oxidase Soluble Domain[J]. Chem. J. Chinese Universities, 2003, 24(10): 1733.

[1]	LI Yidi, TIAN Xiaochun, LI Junpeng, CHEN Lixiang, ZHAO Feng. Electron Transfer on the Semiconductor-microbe Interface and Its Environmental Application [J]. Chem. J. Chinese Universities, 2022, 43(6): 20220089.
[2]	XU Dandan, ZOU Xiucheng, LUO Jing, LIU Ren. Synthesis and Characterization of Phenothiazine-based Schiff Bases as Visible Light Photoinitiators [J]. Chem. J. Chinese Universities, 2022, 43(4): 20210857.
[3]	XU Fei, LI Gangmei, HAN Songde, WANG Guoming. Photochromism and Photomagnetism in Two Dinuclear Lanthanide Complexes [J]. Chem. J. Chinese Universities, 2022, 43(1): 20210337.
[4]	ZHU Min, ZHANG Xiao, YOU Shuli. Visible-light-promoted Dearomatization of Benzene and Derivatives^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1407.
[5]	CHENG Na, HE Yuanyuan, ZHAO Jianwei. Effect of Hetero-atoms on the Electron Transport Behavior in Conjugated Fused Heterocycles [J]. Chem. J. Chinese Universities, 2018, 39(2): 277.
[6]	MING Meijun, BI Tingjun, LI Xiangyuan. Nonequilibrium Solvation Theory Based on Constrained Equilibrium Principle and Its Applications^† [J]. Chem. J. Chinese Universities, 2015, 36(11): 2256.
[7]	LUO Yanling, LIU Yajun. Role of Superoxide in Bioluminescence^† [J]. Chem. J. Chinese Universities, 2015, 36(1): 24.
[8]	LIANG Fangyuan, WU Ranran, CAO Changli, ZHENG Yue, YANG Zhaohui, ZHAO Feng. Research on Extracellular Electron Transfer of Acidithiobacillus Ferrooxidans^† [J]. Chem. J. Chinese Universities, 2014, 35(2): 372.
[9]	ZHANG Kai, DING Chunmei, LIU Huan, ZHU Ying, JIANG Lei. Shewanella-mediated Synthesis of Reduced Graphene Oxide Film for Enhanced Extracellular Electron Transfer^† [J]. Chem. J. Chinese Universities, 2014, 35(10): 2201.
[10]	QIAN Tao, WANG Juan-Juan, ZHANG Qing-Hua, ZHAN Xiao-Li, CHEN Feng-Qiu. Synthesis and Characterization of Gradient Copolymer Composed of [N-Methyl-perfluorohexane-1-sulfonamide] Ethyl Acrylate and MMA [J]. Chem. J. Chinese Universities, 2013, 34(3): 703.
[11]	ZHU Xiao-Qing, YANG Jin-Dong. Fundamental Flaw of Marcus Theory [J]. Chem. J. Chinese Universities, 2013, 34(10): 2247.
[12]	LIU Wei-Qing, LIANG Zhong-Guan, KOU Dong-Xing, HU Lin-Hua, DAI Song-Yuan. Electron Distribution and Contact Interface Transfer Process in Bilayer TiO₂ Films of Dye Sensitized Solar Cell [J]. Chem. J. Chinese Universities, 2012, 33(12): 2697.
[13]	GU Guang-Zhe, DONG She-Ying, HUANG Ting-Lin, TANG Hong-Sheng, ZHENG Jian-Bin. NaNO₂ Biosensor Based on Hollow Nano-nickel Oxide Microsphere and Ionic Liquid Composite Film Immobilizing Hemoglobin [J]. Chem. J. Chinese Universities, 2012, 33(09): 1926.
[14]	LIU Wei-Qing, KOU Dong-Xing, HU Lin-Hua, DAI Song-Yuan. Mechanisms of Electron Transfer and Dynamic Process at the Multiphase Interface in Dye Sensitized Solar Cell [J]. Chem. J. Chinese Universities, 2012, 33(09): 2051.
[15]	SHAO Xiao-Na, ZHANG Xian-Fu. Self-assembly of Phthalocyanine-carbon Nanotubes Dispersed in Micelle and Light Induced Long-lived Charge Separation State [J]. Chem. J. Chinese Universities, 2012, 33(04): 806.

Effect of Val162 Mutation on Structure and Function of Cytochrome c Oxidase Soluble Domain

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments