A Biosensor for Nucleic Acid Hybridization Using 2,3,5-Triphenyl-tetrazoliam Chloride as Electrochemical Intercalator

Abstract

Abstract: Asensitive electrochemical biosensor is reported for the detection of nucleic acid hybridization and sequence-specific DNAs. The biosensor is based on a graphite electrode modified ssDNAusing 2,3,5-triphenyl-tetrazolium chloride(TTC) as hybridization indicator. TTCshows high electroactivity and low background current in electrochemical measurements. It binds to dsDNAs on the electrode surface with high selectivity. The reductive current of intercalated TTCis proportional to the concentration of target ssDNAin the range of 6.0×10^-6—2.0×10^-8 mol/Lin cyclic voltammetry. The detection limit of the biosensor for DNAis 6.0×10^-11 mol/Lin differential pulse voltammetry.

Key words: DNA, 2,3,5-Triphenyl-tetrazolium chloride, Hybridization, Voltammetry

CLC Number:

O657.15

TrendMD:

CHENG Qiong, PENG Tu-Zhi. A Biosensor for Nucleic Acid Hybridization Using 2,3,5-Triphenyl-tetrazoliam Chloride as Electrochemical Intercalator[J]. Chem. J. Chinese Universities, 2002, 23(9): 1680.

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A Biosensor for Nucleic Acid Hybridization Using 2,3,5-Triphenyl-tetrazoliam Chloride as Electrochemical Intercalator

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