Ordered Ring Assembly of Cadmium-Calcein Complex Directed Under the Capillary Flow on the Glass Slide Supports and Its Digitalized Fluorescence Image Analysis

Abstract

Abstract: Afluorescent ordered ring assembly was observed under a fluorescence microscope, and the assembly image was captured by using a digital CCDcamera for analytical purposes. When a droplet of diluted solution of cadmium-calcein complex in 0.01 mol/Lhexahydropyridine was spotted onto the surface of dimethyl dichlorosilane modified glass slide, a symmetrical ordered ring was formed with an outer diameter of 1.10 mm and a ring line width of 0.048 mm. It was found that the fluorescent molecules on the ring line followed a Gaussian distribution, and with which a quantitative equation was established. For a 0.1 μLdroplet of the calcium calcein complex, the maximal fluorescence intensity of the ring is proportional to the content of calcium over the range of 3.0×10^-15～1.0×10^-13mol, and the limit of detection is 2.56×10^-16mol. The proposed method has been successfully applied to the quantification of Cd²⁺ in water samples of Jialingjiang River.

Key words: Cadmium, Calcein, Capillary flow, Fluorescence image analysis

CLC Number:

O675.31

TrendMD:

HUANG Cheng-Zhi, LI Yuan-Fang . Ordered Ring Assembly of Cadmium-Calcein Complex Directed Under the Capillary Flow on the Glass Slide Supports and Its Digitalized Fluorescence Image Analysis[J]. Chem. J. Chinese Universities, 2002, 23(6): 1038.

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Ordered Ring Assembly of Cadmium-Calcein Complex Directed Under the Capillary Flow on the Glass Slide Supports and Its Digitalized Fluorescence Image Analysis

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