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Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (4): 624.

• Articles • Previous Articles     Next Articles

△6 Desaturase Catalyzing Linoleic Acid to γ-Linolenic Acid

XIAN Mo1, KANG Yi-Jian2, YAN Ji-Chang3, LIU Yan1, LI Wen-Xing1, BI Ying-Li1, ZHEN Kai-Ji1   

  1. 1. College of Chemistry. Jilin University, Changchun 130023, China;
    2. Department of Chemical Engineering, Qingdao Institute of Chemical Engineering, Qingdao 266042, China;
    3. Department of Chemistry, Northeast Normal University, Changchun 130024, China
  • Received:2001-03-26 Online:2002-04-24 Published:2002-04-24

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Abstract: The biosynthesis of γ-linolenic acid from linoleic acid with △6 desaturase, Morierella isabellina,is reported.Various factors affecting this process were studied.The results show that the yield of the γ-linolenic acid increases with the decrease of the reaction temperature and reachs a maximum when incubat-ed below 10℃.Adding malate to the reaction mixture caused a marked increase of γ-linolenic acid yield;an increased linolenic acid yield could also be obtained with the increase of pHvalue of the reaction mixtureand reached the maximum at pH 7-8.Oxygen was an essential substrate, and NADPH, CoA and ATP were inpartent cofactors for the reaction.The yield of γ-linolenic acid reached 0.21 mg/mLunder the opti-mal reaction conditions.

Key words: △6 Desaturase, γ-Linolenic acid, Biosynthesis, Mortierella isabellina

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