Studies on the Fluorometric Method for Determining Activity of Catalase and Its Application to Marine Biosamples

Abstract

Abstract: A new promising method for determining activity of catalase and its application are presented. The measurement of catalase activity is based on the inhibition of catalase on Fenton reaction. Catalase catalyzes the dissociation of H₂O₂, one of the main reactants of Fenton reaction which yields ^·OH stoichiometrically, and restrains the increment of the fluorescence intensity, produced as a result of quinoline hydroxylation. The decrease of fluorescence intensity was linearly related to the activity of catalase and the linear range covered from 1.7×10^-3 U/mL to 1.7×10^-2 U/mL. A detection limit of 8.5×10^-4 U/mL catalase was obtained. This method has been applied to the detection of catalase in marine biosamples and satisfactory results were obtained.

Key words: Catalase determination, Quinoline, Fluorometry, Fenton reaction, Biosamples

CLC Number:

O657

TrendMD:

MAO Yu-Xia, ZHENG Hong, GUO Xiang-Qun, CHEN Rong, ZHENG Wei-Yun . Studies on the Fluorometric Method for Determining Activity of Catalase and Its Application to Marine Biosamples[J]. Chem. J. Chinese Universities, 2002, 23(10): 1864.

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Studies on the Fluorometric Method for Determining Activity of Catalase and Its Application to Marine Biosamples

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