Effect of Chelate Reagents on Terbium-apotransferrin (Ⅱ)——The Properties of Anions Binding to Terbium-apotransferrin

Abstract

Abstract: The removal of terbium from terbium apotransferrin by the chelate reagents, EDTA, HEDTA, CDTA, CIT, NTA, EDDA, IDAand SUC, were studied in 0.1mol/L N-(2-hydroxyethyl) piperazine-N'-2-ethanesulfonic acid and pH7.4 using UVdifferential spectra and fluorescence spectroscopies. Terbium fluorescence at 549 nm can be used to monitor the change from terbium-apotransferrin to terbium chelate. In the process of terbium removal there exists an intermediate, which is a ternary complex formed from terbium-apotransferrin (Tb-CapoTf) and the chelate reagent (L). When Lis a synergistic anion, the ternary complex is L-Tb-CapoTf while the ternary complex is Tb-CapoTf-Lwhen Lis a nonsynergistic anion. For the removal of terbium L-Tb-CapoTf is more effective than Tb-CapoTf-L. HEDTA, EDTA, NTAcan make Tb-CapoTf change into Tb-Lor Tb-L₂ completely.

Key words: Serum transferrin, Terbium ion, UV differential spectroscopy, Fluorescence spectroscopy

CLC Number:

O614

TrendMD:

YANG Bin-Sheng, LI Ying-Qi . Effect of Chelate Reagents on Terbium-apotransferrin (Ⅱ)——The Properties of Anions Binding to Terbium-apotransferrin[J]. Chem. J. Chinese Universities, 2001, 22(7): 1086.

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Effect of Chelate Reagents on Terbium-apotransferrin (Ⅱ)——The Properties of Anions Binding to Terbium-apotransferrin

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