Synthesis and Characterizations of New Supports for Enzymes Immobilization from Poly(vinylene carbonate) and α-(2-Aminoethyleneamino)-ω-(2-aminoethyleneamino)-poly(ethylene oxide)

Abstract

Abstract: In this paper, poly(ethylene oxide) end capped with amine groups(PEO) was prepared and dissolved with poly(vinylene carbonate)(PVCA) in DMFsolution. The solution was suspended in paraffin oil to carry out the crosslinking reaction and produce beadlike hydrophilic supports containing reactive cyclic carbonate groups for enzyme immobilization. In above reaction, PEOwas acted as both crosslinking agent and hydrophilic modifier of poly(vinylene carbonate). Evaluation of the supports using trypsin as a model enzyme shows that the specific activity of immobilized trypsin and amount of enzymes coupled to supports were related to PVCA/PEOratio, reaction time, concentration and pHvalue of enzyme solution, etc.. The amount of trypsin coupled and the specific activity reach their maximum at w (PVCA)/ w (PEG)=1/2. The optimal pHvalue for trypsin immobilized increases as compared with the optimal pHvalue for native trypsin. The K_m value of immobilized and native trypsin was determined to be 325 mmol/Land 152 mmol/L, respectively.

Key words: Poly(ethylene glycol), Poly(vinylene carbonate), Fixed enzyme, Support, Trysin

CLC Number:

O631

TrendMD:

HUANG Jia-Xian, DING Lun-Han, CAO Zhe, LI Yue, JIANG Yan . Synthesis and Characterizations of New Supports for Enzymes Immobilization from Poly(vinylene carbonate) and α-(2-Aminoethyleneamino)-ω-(2-aminoethyleneamino)-poly(ethylene oxide)[J]. Chem. J. Chinese Universities, 2001, 22(4): 678.

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Synthesis and Characterizations of New Supports for Enzymes Immobilization from Poly(vinylene carbonate) and α-(2-Aminoethyleneamino)-ω-(2-aminoethyleneamino)-poly(ethylene oxide)

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