Chemical Modification of Human Plasminogen

Chem. J. Chinese Universities ›› 2001, Vol. 22 ›› Issue (4): 577.

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Chemical Modification of Human Plasminogen

LIANG Fang¹, WU Yi-Hui¹, CAO Hai-Shi¹, GUO Hui-Yun¹, LIU Lan-Ying¹, SUN Hong², CHEN Wei-Jin², LIU Shu-Ying²

1. College of Life Science, Jilin University, Changchun 130023, China;
2. Changchun Institute of Biological Products, Changchun 130061, China

Received:2000-03-28 Online:2001-04-24 Published:2001-04-24

Abstract

Abstract: The chemical modification of human plasminogen(HPg) was studied with NAI, 1,2-cyclohexanodine, Ch-T, and EDCas the modification reagents on its Tyr, Arg, Met and carboxyl groups. The results indicate that Tyr and Arg residues are not activity dependent groups, while Met and carboxyl groups played an important role on HPg's activity. The kinetic analysis demonstrates that one Met residue and two carboxyl groups are essential for HPg's activity.

Key words: Human Plasminogen, Chemical modification, Carboxyl group, Methionine

CLC Number:

Q556

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LIANG Fang, WU Yi-Hui, CAO Hai-Shi, GUO Hui-Yun, LIU Lan-Ying, SUN Hong, CHEN Wei-Jin, LIU Shu-Ying . Chemical Modification of Human Plasminogen[J]. Chem. J. Chinese Universities, 2001, 22(4): 577.

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Chemical Modification of Human Plasminogen

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