Studies of Soluble Human Catalytic Antibody with Glutathione Peroxidase Activity

Chem. J. Chinese Universities ›› 2001, Vol. 22 ›› Issue (12): 1976.

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Studies of Soluble Human Catalytic Antibody with Glutathione Peroxidase Activity

QI XiaoJie¹, WANG Lin¹, FANG Fang¹, YAN XiYun², DING Lan¹, ZHAO DaQing¹, NI JiaZuan¹

1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun3002 China;
2. Institute of Microbiology, Chinese

Received:2000-12-22 Online:2001-12-24 Published:2001-12-24

Abstract

Abstract: By screening the phagedisplayed human single chain antibody library, we have got the specific single chain antibody bound to GSHSDNPbutyl ester as the hapten. The tertiary structure of the protein was analyzed with the aid of computer, and the results showed the CDR3 region located on the surface of the antibody. The soluble antibody was expressed in E.coli. and the active site serine was converted into selenocysteine with the chemical modifying method, which resulted in the catalytic antibody with GPx activity of 80 U/μmol. Furthermore, the same PingPong mechanism as the natural GPx was observed when the kinetic behavior of the antibody was studied.

Key words: Glutathione peroxidase, Human catalytic antibody, Soluble expression

CLC Number:

O622

TrendMD:

QI XiaoJie, WANG Lin, FANG Fang, YAN XiYun, DING Lan, ZHAO DaQing, NI JiaZuan. Studies of Soluble Human Catalytic Antibody with Glutathione Peroxidase Activity[J]. Chem. J. Chinese Universities, 2001, 22(12): 1976.

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Studies of Soluble Human Catalytic Antibody with Glutathione Peroxidase Activity

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