Desorption-ionization Mass Spectrometry with Porous Silica Gel and Its Derivatives as Matrix

Abstract

Abstract: Porous silica gel and its derivatives have been applied as matrix for the analysis of small molecules. In contrast to the conventional MALDI TOF MS, the signal interference of low molecular analytes by the matrix has been eliminated in the MALDI-TOF-M-Swith porous silica gel and its derivatives as the matrix. The detection of limit by this method reached the fetomole levels, and almost no fragment of the analytes has been observed. The porous structure of silica gel is essential for effective desorption and ionization of the analytes. Among several different types of silica gel, the silica gel with the pore size of 30 nm gives the best signal. The analyte penetration into the pore is very important. Matrix agents of DHBand CCAhave been covalently bound to silica gel for the improvement of the intensity of the spectrum signal.

Key words: Silica gel, Matrix, Desorption ionization mass spectrometry, Immobilization of matrix, Small molecule analysis

CLC Number:

O657

TrendMD:

ZHANG Qing-Chun, ZOU Han-Fa, GUO Zhong, CHEN Xiao-Ming, JIANG Hong-Hai, NI Jian-Yi . Desorption-ionization Mass Spectrometry with Porous Silica Gel and Its Derivatives as Matrix[J]. Chem. J. Chinese Universities, 2001, 22(10): 1637.

[1]	LI Weitang, REN Jiajun, SHUAI Zhigang. Theory and Applications of Time Dependent Density Matrix Renormalization Group [J]. Chem. J. Chinese Universities, 2021, 42(7): 2085.
[2]	QIN Chunping, WANG Xianliu, TANG Han, YI Bingcheng, LIU Chang, ZHANG Yanzhong. Osteogenesis-promoting Effects of the Electrospun Nanofibers Containing Decellularized Bone Matrix ^† [J]. Chem. J. Chinese Universities, 2020, 41(4): 780.
[3]	JIANG Tong, REN Jiajun, SHUAI Zhigang. Frequency Domain Density Matrix Renormalization Group [J]. Chem. J. Chinese Universities, 2020, 41(12): 2610.
[4]	HOU Junying, HAO Jianjun, WANG Yaya, LIU Jingchun. Fabrication of Cu₃(BTC)₂ Mixed Matrix Membrane and Its Application in Continuous Flow Process ^† [J]. Chem. J. Chinese Universities, 2019, 40(9): 1926.
[5]	WANG Ning,ZHU Huifang,WANG Lu,ZHANG Tiantian,GU Jiali,SHU Jie. Structural Identification and Asymmetric-exchange Dynamics Study of Esomeprazole Magnesium in Specific Solution as Probed by Using ¹H NMR Spectra^† [J]. Chem. J. Chinese Universities, 2018, 39(9): 1919.
[6]	TANG Keyun,LI Luoyuan,FU Limin,AI Xicheng,ZHANG Jianping. Effect of Crystal Matrix on Energy Transfer Mechanism in Rare-earth Upconversion Nanomaterials^† [J]. Chem. J. Chinese Universities, 2018, 39(10): 2136.
[7]	YANG Caihong, MAN Chunli, XUE Wanlei, WANG Ting, CHEN Di, CHEN Qian, WU Liguang. Fabrication of TiO₂-GO/PI Mixed Matrix Membranes by In-situ Polymerization and Their Gas Permeation Property^† [J]. Chem. J. Chinese Universities, 2017, 38(4): 686.
[8]	MAHMUTJAN Jelil, ABLAT Mamat, ALIMJAN Abaydulla, MAMAT Tursun. Investigation of Topology-distinct Stable Structures for Hydrogen Fluoride Clusters (HF)_n(n=2—8) by the Methods of Graph Theoretical Enumerations and Quantum Chemical Calculations^† [J]. Chem. J. Chinese Universities, 2016, 37(12): 2275.
[9]	XU Lanlan, ZHAO Qiqi, YU He, WANG Jingchen, WANG Huijun, YANG Qin, ZHU Huajie, LI Yan. Absolute Configuration Determination of One New Compound Trichoderol A from Trichoderma sp. Fungus^† [J]. Chem. J. Chinese Universities, 2016, 37(11): 1972.
[10]	XU Buyi, YE Yi, RUAN Ruoyun, YAN Youyi, LIAO Linchuan. Determination of Illegal Drugs in Urine Using Excitation-emission Matrix Fluorescence Coupled with Second-order Calibration^† [J]. Chem. J. Chinese Universities, 2015, 36(9): 1667.
[11]	NIE Guizhen, LI Laisheng, CHENG Biaoping, ZHOU Rendan, ZHANG Hongfu. Enantioseparations of Dihydropyridine Drugs by Sulfobutyl Ether-β-Cyclodextrin Modified Capillary Electrochromatography^† [J]. Chem. J. Chinese Universities, 2014, 35(7): 1414.
[12]	DUAN Cuijia, CAO Yiming, JIE Xingming, WANG Lina, YUAN Quan. Preparation and Gas Separation Properties of Metal-organic Frameworks/Polyimide Mixed Matrix Membranes^† [J]. Chem. J. Chinese Universities, 2014, 35(7): 1584.
[13]	SHANG Guangyuan, LI Ming. Preparation and Properties of Surface Modified Nano-Ni Particles with n-Hexadecylphosphonic and Its Composites [J]. Chem. J. Chinese Universities, 2014, 35(2): 427.
[14]	ZHAO Chaoyue, WANG Na, WANG Juanjing, HE Xi, LI Jiang. Construction and Functional Detection of Mutant Plasmid EMMPRIN Phosphorylation^† [J]. Chem. J. Chinese Universities, 2014, 35(10): 2104.
[15]	XU Rui-Feng, BAI Yun-Peng, LIAN Wen-Hui, LIU Shu, SONG Feng-Rui, LIU Zhi-Qiang, LIU Shu-Ying. Tungstate-containing Clusters Ions in the Gas Phase by MALDI-FTICRMS [J]. Chem. J. Chinese Universities, 2013, 34(5): 1106.

Desorption-ionization Mass Spectrometry with Porous Silica Gel and Its Derivatives as Matrix

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments