Studies on the Packings with Guanido of Affinity Chromatography for the Separation of Urokinase

Chem. J. Chinese Universities ›› 2000, Vol. 21 ›› Issue (5): 737.

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Studies on the Packings with Guanido of Affinity Chromatography for the Separation of Urokinase

CHANG Jian-Hua¹, GAO Jun-Ping¹, LIANG Feng¹, FENG Yue¹, DONG Yu-Sheng¹, SU Tian-Sheng², GUO Li-An³

1. Department of Chemistry, Northwest University, Xian 710069, China;
2. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China;
3. Central Laboratory, the Fourth Military University, Xi'an 710032, China

Received:1999-07-13 Online:2000-05-24 Published:2000-05-24

Abstract

Abstract: Five guanido packings of affinity chromatography for the separation of urokinase have been synthesized. The separation characteristics and the effect of matrix and ligands on them have been studied. The guanidine derivative is a fine ligand, the packings with ligand of arginine are much better than that with ligand of p-ABZ. The polyepoxypropyl methacrylate microsphere is a good matrix. The comprehensive function of packings with ligand of arginine and matrix of polyepoxypropyl methacrylate microsphere is more excellent than Sepharose-p-ABZ.

Key words: Packings, Affinity chromatography, Separation, Urokinase

CLC Number:

O621.29

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CHANG Jian-Hua, GAO Jun-Ping, LIANG Feng, FENG Yue, DONG Yu-Sheng, SU Tian-Sheng, GUO Li-An. Studies on the Packings with Guanido of Affinity Chromatography for the Separation of Urokinase[J]. Chem. J. Chinese Universities, 2000, 21(5): 737.

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Studies on the Packings with Guanido of Affinity Chromatography for the Separation of Urokinase

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