Immobilization of Antigen and Antibody on Gold Surface and Determination Using Fluorescent Method

Abstract

Abstract: A Gold surface was modified with N-acetylcysteine to produce a self-assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS). The competitive method(Ⅰ), the sandwich method(Ⅱ) and the surplus method(Ⅲ) were developed to determine rabbit IgG or goat anti rabbit IgG, respectively. A luminescence analyzer connected with a fluorescence microscope was employed to detect the light intensity of labeled sheep anti rabbit IgG. The results of sensitivity and dynamic range were obtained as 25 mg/L and 25—600 mg/L for method(Ⅰ), 1.0 mg/L and 1—200 mg/L for method(Ⅱ) and 25 mg/L and 25—500 mg/L for method(Ⅲ), respectively.

Key words: Immunosensor, Self-assembled monolayer, Fluorescence

CLC Number:

O657

TrendMD:

JIANG Xiong-Ping, XU Dan-Ke, LIU Yao-Qing, MA Li-Ren. Immobilization of Antigen and Antibody on Gold Surface and Determination Using Fluorescent Method[J]. Chem. J. Chinese Universities, 2000, 21(11): 1664.

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Immobilization of Antigen and Antibody on Gold Surface and Determination Using Fluorescent Method

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