Abstract: Amolecularly templated synthetic polymer receptor highly selective for trimethoprim was prepared by a molecular selfassembly imprinting technique. The selective binding characteristics of the template polymer were evaluated by Scatchard analysis, ¹HNMR study and Ultraviolet spectrometry. The results showed that a class of same binding sites, which could specificially bind to trimethoprim by cooperative hydrogen bonding, were formed in the polymer, and its dissociation constant was estimated to be 2.22×10^-4mol/L. The selective binding experiments for substrates indicated that the polymer gave much higher affinity and selectivity for trimethoprim than for sulfanilamide and sulfapyridine. It is possiple to be a good adsorption and binding materiaLIn the selective enrichment and determination of trace trimethoprim in complex biosamples.

Key words: Molacular selfassembly imprinting technique, Template polymer, Trimethoprim, Substrate selectivity