Identification of Angiogenesis Inhibitory Factor by HPLC and ESIMS

Abstract

Abstract: The studies of SCAIF-I(an unknown protein) by HPLC and ESIMS was reported in this paper. The SCAIF Iis a kind of antiangiogenesis extracted from shark cartilage. The extracted protein was purified by HPLC and then studied by ESIMS. The molecule weight of the SCAIF-I protein was confirmed to be 15601 in the first time. Peptide map of the unknown protein was obtained by HPLC-ESIMS with a trypsin digestion and possible partial sequences were deduced with the online CIDtechnique. With partial sequence information the homologous proteins are searched with protein database. The homology of SCAIF-I summarized in this work is usefuLIn further studies of this unknown protein.

Key words: HPLC, ESIMS, SCAIF

CLC Number:

O657.6

TrendMD:

YUE Gui-Hua, YANG Peng-Yuan, SHEN Xian-Rong, JI Dong-Mei, REN Da-Ming, LIU Wei-Ping, XU Yun-Min, WANG Hong-Hai . Identification of Angiogenesis Inhibitory Factor by HPLC and ESIMS[J]. Chem. J. Chinese Universities, 1999, 20(12): 1868.

[1]	PAN Mengyun, FENG Liuxing, LI Hongmei. Absolute Quantification of Haemoglobin in Whole Blood by Species-unspecific Isotope-dilution Liquid Chromatography-inductively Coupled Plasma Mass Spectrometry [J]. Chem. J. Chinese Universities, 2020, 41(9): 1983.
[2]	WANG Tianqi,YU Qiongwei,FENG Yuqi. Analysis of Imidazole Propionic Acid in Serum of Patients with Type 2 Diabetes Based on NiO@SiO₂ Solid-phase Extraction Coupled with Liquid Chromatography-Mass Spectrometry ^† [J]. Chem. J. Chinese Universities, 2020, 41(2): 262.
[3]	ZHAO Mengxin, MENG Zhe, LI Heping, MA Zongqin, ZHAN Haijuan, LIU Wanyi. Photodegradation of Antibiotic in Environmental Water by Graphene Oxide Modulation Bismuth Molybdate Under Visible Light Irradiation [J]. Chem. J. Chinese Universities, 2020, 41(11): 2479.
[4]	ZHAO Huanxi,WANG Qiuying,SUN Xiuli,LI Xue,MIAO Rui,WU Dongxue,LIU Shuying,XIU Yang. Discrimination of Ginseng Origins and Identification of Ginsenoside Markers Based on HPLC-MS Combined with Multivariate Statistical Analysis^† [J]. Chem. J. Chinese Universities, 2019, 40(2): 246.
[5]	MIAO Rui,WU Dongxue,WANG Qiuying,ZHAO Huanxi,LI Xue,XIU Yang,LIU Shuying. Rapid Separation of Ginsenosides Based on Multi-walled Carbon Nanotubes^† [J]. Chem. J. Chinese Universities, 2018, 39(10): 2178.
[6]	LI Nan, ZHAO Huanxi, LI Jing, WANG Nan, YU Bohao, YUE Hao, YU Shanshan. Transformation of Total Notoginsenosides by Recombinant Endocellulase Fpendo5A^† [J]. Chem. J. Chinese Universities, 2017, 38(12): 2185.
[7]	LI Xue, ZHAO Huanxi, MIAO Rui, LI Wenying, XIU Yang, LIU Shuying. Structure and Pathway Research on Chemical Transformation of Ginsenoside Rb₁ via HPLC-HRMS/MSⁿ/QqQ Technique^† [J]. Chem. J. Chinese Universities, 2017, 38(10): 1730.
[8]	PI Zifeng, WANG Qianqian, ZHANG Jing, Song Fengrui, LIU Zhiqiang. Effect of Schisandra Fruit on Neurochemicals in Hippocampus of Diabetic Encephalopathy Rat Using Online MD-HPLC-MS/MS^† [J]. Chem. J. Chinese Universities, 2015, 36(3): 442.
[9]	ZHANG Yongpo, ZHANG Yang, SUN Huailin. Stereo Selective Synthesis and Absolute Configuration Determination of (2E,4S)-4-(t-butoxycarbonylamino)-5-[(3S)-2'-oxo-3'-pyrrolidinyl]-2-pentenoic Acid Ethyl Ester^† [J]. Chem. J. Chinese Universities, 2014, 35(2): 292.
[10]	YAO Hua, JIN Yongri, YANG Jie, LI Lanjie, SUN Ting, SHI Xiaolei, LI Xuwen. Conversion Rule of Rare Ginsenosides Produced from Major Ginsenosides by Confined Microiwave Promoted Degradation Method^† [J]. Chem. J. Chinese Universities, 2014, 35(11): 2317.
[11]	LI Lai-Sheng, CHEN Hong, MA Hai-Ping, FANG Yi-Shan. Preparation, Characterization and Evaluation of Magnolol-bonded Silica Gel Stationary Phase for Reversed-phase Liquid Chromatography [J]. Chem. J. Chinese Universities, 2012, 33(04): 707.
[12]	WANG Wei-Hua, LIU Chun-Hong, SUN Yuan-Ming, LIN Feng, ZHANG Ming-Ming, WANG Zhao-Bin, ZHANG Min-Yi. Metabolic Profiles of Nonylphenol in Rat Urine [J]. Chem. J. Chinese Universities, 2011, 32(10): 2280.
[13]	WANG Ying, XIAO Yao, REN Rui-Bing, BAO Chang-Li, ZHANG Han-Qi, YU Ai-Min, .... Homogeneous Ionic Liquid Microextraction of Fleroxacin and Sulfaquinoxaline from Water Samples [J]. Chem. J. Chinese Universities, 2010, 31(9): 1721.
[14]	ZHANG Ming-Shan, LIU Guo-Zhu, LI Sheng-Jun, CHEN Bo*, YAO Shou-Zhuo. Solvent Induced Phase Transition Extraction Method for High Performance Liquid Chromatography-Mass Spectrometry Analysis of Plasma Samples of Pretreatment [J]. Chem. J. Chinese Universities, 2010, 31(8): 1517.
[15]	DU Qin-Qin, ZHANG Xu, SONG Feng-Rui, LIU Zhi-Qiang, LIU Shu-Ying. HPLC-ESI-MS Analysis of Ginsenosides and Antioxidant Activity During Combination of Ginseng with Aconitum Carmichaeli* and Coptis Chinensis [J]. Chem. J. Chinese Universities, 2010, 31(7): 1332.

Identification of Angiogenesis Inhibitory Factor by HPLC and ESIMS

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments