Studies on Components and Molecular Weights of Polysaccharides from Lentinus Edodes

Abstract

Abstract: Four heteropolysaccharides(L-FⅠ_L-FⅣ_n ) and two glucans(L-FⅤ and L-FⅥ_n ) were isolated from the fruit bodies of lentinus edodes by using aqueous 09% NaCl, 85% ethanol, hot water(120 ℃, high pressure), aqueous 1% ammonium oxalate, aqueous 5% NaOH/005% NaBH₄ and 2% NaOH/2% urea, respectively. The results analyzed with IRand HPLCshowed that the heteropolysaccharides consist of Dglucose, glucuronic acid, xylose, mannose and galactose and rhamnose, and the D-glucose content in the polysaccharides increases with the progress of isolation process. The weight average molecular weights M_w, root-mean-square radii of gyration 〈S²〉^1/2, second Virial coefficients A₂ and number average molecular weights M_n of the polysaccharides were studied by using light scattering and membrane osmometry. The M_w values of the samples of L-FⅡ, L-FⅢ, L-FⅤ and L-FⅥ n are 19.7×10⁴, 19.23×10⁴, 13.64×10⁴ and 13.67×10⁴, respectively. The polydispersity indexes of the polysaccharides M_w/ M_n lie in the range of 3～5.

Key words: Lentinan edodes, Molecular weight, HPLC, Light scattering, Membrane osmometry

CLC Number:

O636.1

TrendMD:

ZHANG Li-Na, ZHANG Ping-Yi, LI Xiang, PAN Su, ZHU Rong-Ping . Studies on Components and Molecular Weights of Polysaccharides from Lentinus Edodes[J]. Chem. J. Chinese Universities, 1998, 19(9): 1513.

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Studies on Components and Molecular Weights of Polysaccharides from Lentinus Edodes

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