Abstract: Anovel synthesis of acridinium ester as a chemiluminescent immunoassay reagent was designed in this paper. This novel synthesis involved the production of acridine-9-carboxylic acid from diphenylamine, the acridine-9-carboxylic acid was converted to acridine-9-carbonylchloride, and reacted with 4-(2-Nsuccinimdyloxycarbonylethyl) phenol, to produce 4-(2-succinimdyloxycarbonylethyl) phenyl-9-acridine carboxylate, this is followed by conversion to 4-(2-succinimdyloxycarbonylethyl) phenyl 10-methylacridinum-9-carboxylate fluorosulfonate(so called acridinium ester).

Key words: 4-(2-succinimdyloxycarbonylethyl) phenyl-10-methylacridinum-9-carboxylate fluo rosulfonate, Chemiluminescent immunoassay, Synthesis, Diphenylamine