The Gradient Distribution of Mobile Phase in HPLC

Chem. J. Chinese Universities ›› 1998, Vol. 19 ›› Issue (10): 1556.

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The Gradient Distribution of Mobile Phase in HPLC

LI Rui-Jiang, ZHANG Wei-Bing, NI Jian-Yi, ZOU Han-Fa, ZHANG Yu-Kui

National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116011

Received:1997-12-12 Online:1998-10-24 Published:1998-10-24

Abstract

Abstract: In the gradient elution of HPLC, the delay and dispersion of mobile phase during the transmission of mobile phase in column, and the out column effect result in that, there is a gradient distribution in the variation of mobile phase content with time at any location in column, and it obeys a distribution law. In this paper, the law is discussed theoretically and experimentally, and the effect on the law of instrumental condition and different solvents is also discussed.

Key words: HPLC, Gradient elution, Distribution law

CLC Number:

O657

TrendMD:

LI Rui-Jiang, ZHANG Wei-Bing, NI Jian-Yi, ZOU Han-Fa, ZHANG Yu-Kui . The Gradient Distribution of Mobile Phase in HPLC[J]. Chem. J. Chinese Universities, 1998, 19(10): 1556.

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The Gradient Distribution of Mobile Phase in HPLC

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