Studies on Indirect Thermo-Optical Detection for Capillary Electrophoresis

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (8): 1182.

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Studies on Indirect Thermo-Optical Detection for Capillary Electrophoresis

REN Ji-Cun¹, LI Bin-Cheng¹, DENG Yan-Zhuo², CHENG Jie-Ke¹

1. Department of Chemistry, Wuhan University, Wuhan, 430072;
2. Department of Analysis and Testing Science, Wuhan University, Wuhan, 430072

Received:1994-10-12 Revised:1995-04-24 Online:1995-08-24 Published:1995-08-24

Abstract

Abstract: Indirect thermo-optical detection for capillary electrophoresis was presented first by combining indirect detection with thermo-optical method.In this detection,methylene blue solution was used as background electrolyte solution,because it has large molar absorption coefficient at 633 nm. This wavelength matches well with that of He-Ne laser(632.8 nm).The detection was applied to detect amino acids in capillary zone electrophoresis separation. The detection limit for lysine was 5×10^-6mol/L(S/N=2).

Key words: Capillary electrophoresis, Indirect thermo-optical detection, Amino acid, Separation

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REN Ji-Cun, LI Bin-Cheng, DENG Yan-Zhuo, CHENG Jie-Ke . Studies on Indirect Thermo-Optical Detection for Capillary Electrophoresis[J]. Chem. J. Chinese Universities, 1995, 16(8): 1182.

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Studies on Indirect Thermo-Optical Detection for Capillary Electrophoresis

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