Investigation on Carbon fiber Microelectrodes(ⅩⅧ)─The Determination of Hemoglobin at a Methylene Blue/Nafion Modified Carbon Fiber Microcylinder Electrode

Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (6): 827.

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Investigation on Carbon fiber Microelectrodes(ⅩⅧ)─The Determination of Hemoglobin at a Methylene Blue/Nafion Modified Carbon Fiber Microcylinder Electrode

JU Huang-Xian, XUN Yi-Gang, CHEN Hong-Yuan

Department of Chemistry, Nanjing University, Najing, 210008

Received:1993-07-15 Revised:1993-01-30 Online:1994-06-24 Published:1994-06-24

Abstract

Abstract: The methylene blue/Nafion modified carbon fiber microcylinder electrode is manufactured.The state and constitution of the modified electrode surface is observed by scanning electron microscope.The stability is very good in either events scaned with cyclic voltmmetry or immersed in the buffer solution of pH=7.2.The electrode shows significantly electrocatalytic activity to the oxidation of hemoglobin in the system of weak acid, it can be used for the determination of hemoglobin in the range of 5×10^-6-5×10^-5mol/L.The variation coefficient of peak current is 3.5%determining continuously 6×10^-6mol/L HBfor 6 times.The catalytic mechanism is discussed.

Key words: Carbon fiber microelectrode, Microcylinder electrode, Nafion modified electrode, Methylene blue, Hemoglobin

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JU Huang-Xian, XUN Yi-Gang, CHEN Hong-Yuan. Investigation on Carbon fiber Microelectrodes(ⅩⅧ)─The Determination of Hemoglobin at a Methylene Blue/Nafion Modified Carbon Fiber Microcylinder Electrode[J]. Chem. J. Chinese Universities, 1994, 15(6): 827.

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Investigation on Carbon fiber Microelectrodes(ⅩⅧ)─The Determination of Hemoglobin at a Methylene Blue/Nafion Modified Carbon Fiber Microcylinder Electrode

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