Computer-Assisted Optimization of Separation of Amino Acids in Column Chromatography

Abstract

Abstract: A computer-assisted method is presented for optimization of ICand pHselection of mobile phase in column chromatography.The optimization of expected separation is based on a polynomial estimation from 4-5preliminary runs.Satatical scanning technique is used for the optimization and computer simulation system(CSC) is established for chromatographic performance measurement in this method.By separation of three amino acids,excellent agreement is obtained between simulated and experimental results.

Key words: Computer, Optimization of chromatography, Column chromatography

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WANG Qin-Sun, YAN Bing-Wen, WANG Shou-Tin, TAO Xue. Computer-Assisted Optimization of Separation of Amino Acids in Column Chromatography[J]. Chem. J. Chinese Universities, 1994, 15(1): 72.

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Computer-Assisted Optimization of Separation of Amino Acids in Column Chromatography

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