Polydopamine-coated Gold Nanorods for Rat Submandibular Gland Radiography†

doi:10.7503/cjcu20180362

Abstract

Abstract:

We demonstrate the application of polydopamine-coated gold nanorods in the CT imaging of rat submandibular gland. Biocompatible polydopamine was coated on the surface of gold nanorods to lower the toxicity. CT value rises with the increase of the concentration of polydopamine-coated gold nanorods in vitro, which indicates the high sensitivity to X-ray. The polydopamine-coated gold nanorods are further applied to rat submandibular gland in vivo and radiographs are obtained. H&E staining of heart, liver, spleen, lungand kidney show no obvious inflammation for the rats.

Key words: Gold nanorods, Computerized tomography, Submandibular gland, Radiography imaging

CLC Number:

O631

TrendMD:

SONG Ziqi,GONG Baijuan,WANG Lu,FENG Jing,WANG Bo,YAN Hongjing,LI Zhimin,SUN Hongchen. Polydopamine-coated Gold Nanorods for Rat Submandibular Gland Radiography^†[J]. Chem. J. Chinese Universities, 2019, 40(1): 166.

Figures/Tables 7

Fig.1 TEM images(A, B) and UV-Vis-NIR absorption spectra(C, D) of gold nanorods(A, C) and polydopamine coated gold nanorods(B, D)

Fig.2 FTIR spectra of CTAB(a), SDS(b), DA(c), AuNRs(d) and AuNRs@PDA(e)

Fig.3 XRD(A) and XPS(B) spectra of gold nanorods

Fig.4 In vitro contrast enhanced CT imaging of AuNRs@PDA(A1—A6) and CT values of AuNRs@PDA with different concentrations(B)Concentration of AuNRs@PDA/(mg·mL-1): (A1) 0; (A2) 0.25; (A3) 0.5; (A4) 1; (A5) 2; (A6) 3.

Fig.5 Cytotoxicities of gold nanorods and polydopamine coated gold nanorods(A) and CTAB, SDS and DA(B) with concentrations

Fig.6 In vivo submandibular gland imaging with AuNRs@PDA of different concentrationsConcentration of AuNRs@PDA/(mg·mL-1): (A) 0; (B) 0.25; (C) 0.5; (D) 1; (E) 2; (F) 3.In these figures, the arrow points to the position of the submandibular gland.

Fig.7 Histological evaluation of AuNRs@PDA toxicity in vivo in rat submandibular gland(A, A'), heart(B, B'), liver(C, C'), spleen(D, D'), lung(E, E') and kidney(F, F') by H&E staining method(A—F) Control; (A'—F') experiment.

References 32

[1]	Specht L., Berthelsen A. K., Seminars in Nuclear Medicine,2018, 48(1), 67—75
[2]	Bankier A. A., Macmahon H., Goo J. M., Rubin G. D., Schaefer-Prokop C. M., Naidich D. P., Radiology,2017, 285(2), 584—600
[3]	Wile G. E., Leyendecker J. R., Krehbiel K. A., Dyer R. B., Zagoria R. J., Radiographics A Review Publication of the Radiological Society of North America Inc. ,2016, 27(2), 325—340
[4]	Koo H. J., Lim S., Choe J., Choi S. H., Sung H., Do K. H., Radiographics A Review Publication of the Radiological Society of North America Inc,2018, 38(3), 719—739
[5]	Brenner D. J., Mccollough C. H., Orton C. G., Medical Physics,2006, 33(5), 1189—1191
[6]	Lee N., Choi S. H., Hyeon T., Adv. Mater. ,2013, 25(19), 2641—2660
[7]	Yang Q., He Q.Z., Yu F., Zhang Y. L., Si Q. Y.,Medical Journal of Chinese People’s Liberation Army, 2018, (2), 135—139
	(杨琴, 何其舟, 余飞, 张艳丽, 斯光晏. 解放军医学杂志, 2018, (2), 135—139)
[8]	Dizendorf E., Hany T. F., Buck A., von Schulthess G. K., Burger C., Journal of Nuclear Medicine,2003, 44(5), 732—738
[9]	Jung Y., Hwang H. S., Na K., Biomaterials,2018, 160, 15—23
[10]	Kennedy R. A., Br. J. Oral. Maxillofac. Surg., 2018, 56(2), 90—95
[11]	Eisbruch A., Haken R. K. T., Kim H. M., Marsh L. H., Ship J. A., Int. J. Radiat. Oncol. Biol. Phys.,1999, 45(3), 577—587
[12]	Schulz M. D., Weisberger R. D., Oral. Surg. Oral. Med. Oral. Patho.,1948, 1(3), 233—248
[13]	Thomas A. R., Br. J. Radiol., 1956, 29(340), 209—312
[14]	Rubin P., Holt J. F., Am. J. Roentgenol. Radium. Ther. Nucl. Med.,1957, 77(4), 575—598
[15]	Zou Z.J.,Chinese Journal of Stomatology, 1954, (1), 18—22
	(邹兆菊. 中华口腔科杂志, 1954, (1), 18—22)
[16]	Zou Z.J.,Chinese Medical Journal, 1978, (1), 353—357
	(邹兆菊. 中华医学杂志, 1978, (1), 353—357)
[17]	Aframian D. J., Keshet N., Nadler C., Oral. Dis.,2018, 24(1/2), 191—193
[18]	Li W., Wei L., Wang F., Peng S., Cheng Y., Li B., J. Oral. Rehabil.,2018, 45(12), 983—989
[19]	Zou Z.J., Zhang Z. Y., Hua H.,Chinese Journal of Stomatology, 1996, (2), 81—84
	(邹兆菊, 张祖燕, 华红. 中华口腔医学杂志, 1996, (2), 81—84)
[20]	Wu Y. W., Leow K. S., Zhu Y., Tan C. H., Ann. Acad. Med. Singapore,2016, 45(4), 157—164
[21]	Bhat K. N., Arroyave C. M., Crown R., Ann. Allergy,1976, 37(3), 169—173
[22]	Cheng L., Wang C., Feng L., Yang K., Liu Z., Chemical Reviews,2014, 114(21), 10869—10939
[23]	He W., Ai K., Lu L., Science China Chemistry,2015, 58(5), 753—760
[24]	Yang X., Yang M., Pang B., Vara M., Xia Y., Chemical Reviews,2015, 115(19), 10410—10488
[25]	Li J., Jiang F., Yang B., Song X. R., Liu Y., Yang H. H., Cao D. R., Shi W. R., Chen G. N., Scientific Reports,2013, 3(6), 1998—2013
[26]	Lusic H., Grinstaff M. W., Chemical Reviews,2013, 113(3), 1641—1666
[27]	Li B., Ye K., Zhang Y., Qin J., Zou R., Xu K., Huang X., Xiao Z., Zhang W., Lu X., Hu J., Advanced Materials,2015, 27(8), 1339—1345
[28]	Dong K., Liu Z., Liu J., Huang S., Li Z., Yuan Q., Ren J., Qu X., Nanoscale,2014, 6(4), 2211—2217
[29]	Liu Y., Ai K., Lu L., Acc. Chem. Res., 2012, 45(10), 1817—1827
[30]	Liu S., Wang L., Lin M., Wang D., Song Z., Li S., Ge R., Zhang X., Liu Y., Li Z., Sun H., Yang B., Zhang H., ACS Appl. Mater. Interfaces,2017, 9(51), 44293—44306
[31]	Choi C. K. K., Choi C. H. J., Bian L., J. Am. Chem. Soc.,2015, 137(23), 7337—7346
[32]	He W., Wu X., Liu J., Zhang K., Chu W., Feng L., Hu X., Zhou W., Xie S., J. Phys. Chem. C,2009, 113(24), 10505—10510