Chem. J. Chinese Universities ›› 1993, Vol. 14 ›› Issue (12): 1697.

• Articles • Previous Articles     Next Articles

The Research on the Relation of the Activity and Incubation Time of β-GIucuronidase

ZHANG Yi-Zhong1, LIU Xin1, YE Li1, ZHANG Chang-Jiu1, ZHANG You-Jie2   

  1. 1. National Research Institute of Sports Medicine, Beijing, 100029;
    2. Center of Analysis and Testing, Central China Normal Univ., Wuhan
  • Received:1993-02-27 Revised:1993-08-22 Online:1993-12-24 Published:1993-12-24

Abstract: To analyze the urinary steroids with GC-MS, conjugated fraction as usual is first hy- drolyzed with β-Glucuronidase at 55℃ for 3 h or 37℃ for 12 h.This is a time consuming step for performing the routine doping control analysis.In this paper the relation between the hy-drolyzing activity and the incubation time of β-Glucuronidase from E.Coli was studies.It was found that the hydrolyzed was completed within 1 h.

Key words: β-Glucuronidase, Doping, Anabolic Steroid

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