Comparison of the Hydrolyzed Activity of β-Glucuronidases from Different Sources While Determining the Testosterone Level in Doping Control

Chem. J. Chinese Universities ›› 1991, Vol. 12 ›› Issue (7): 918.

• Articles • Previous Articles Next Articles

Comparison of the Hydrolyzed Activity of β-Glucuronidases from Different Sources While Determining the Testosterone Level in Doping Control

Zhang Chang-jiu¹, Zhang Yi-zhong¹, Lin Qiang¹, Ye Li¹, Yang Tian-le¹, Zhang Ji², Liu Chun-sheng², Wang Mu-zou², Zhou Tong-hui², Yang Ze-yi³

1. National Research Institute of Sports Medicine, Beijing, 100029;
2. Institute of Materia Medico, Chinese Academy of Medical Sciences, Beijing;
3. Beijing Medical University, Beijing

Received:1990-03-07 Online:1991-07-24 Published:1991-07-24

Abstract

Abstract: Selected Ion Monitor (SIM) of GC-MS is used to determine the amounts of testosterone (T), epitestosterone(ET) and their ratio in urine.The affection of five β-glucuronidases to the determination of Tlevel has been compared. It is figured out that both the Calf Enzyme (made in China) and the E. Coli(IX-A) from Sigma have strong activities while hydrolyzing the combined T, ETin urine. So they are fit to the determination of Tand ETlevel of urine in doping Control.

Key words: Testosterone, Doping, β-Glucuronidase

TrendMD:

Zhang Chang-jiu, Zhang Yi-zhong, Lin Qiang, Ye Li, Yang Tian-le, Zhang Ji, Liu Chun-sheng, Wang Mu-zou, Zhou Tong-hui, Yang Ze-yi. Comparison of the Hydrolyzed Activity of β-Glucuronidases from Different Sources While Determining the Testosterone Level in Doping Control[J]. Chem. J. Chinese Universities, 1991, 12(7): 918.

[1]	CHENG Qian, YANG Bolong, WU Wenyi, XIANG Zhonghua. S-doped Fe-N-C as Catalysts for Highly Reactive Oxygen Reduction Reactions [J]. Chem. J. Chinese Universities, 2022, 43(9): 20220341.
[2]	SONG Youwei, AN Jiangwei, WANG Zheng, WANG Xuhui, QUAN Yanhong, REN Jun, ZHAO Jinxian. Effects of Ag，Zn，Pd-doping on Catalytic Performance of Copper Catalyst for Selective Hydrogenation of Dimethyl Oxalate [J]. Chem. J. Chinese Universities, 2022, 43(6): 20210842.
[3]	SUN Xuefeng, RENAGUL Abdurahman, YANG Tongsheng, YANG Qianting. Synthesis and Luminescence Properties of Cr，In Co-doped Small Size MgGa₂O₄ Near-infrared Persistent Luminescence Nanoparticles [J]. Chem. J. Chinese Universities, 2022, 43(4): 20210850.
[4]	WANG Zumin, MENG Cheng, YU Ranbo. Doping Regulation in Transition Metal Phosphides for Hydrogen Evolution Catalysts [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220544.
[5]	HONG Yangyu, XING Hongzhu, BING Qiming, GAO Xuwen, QI Bin, CHEN Yakun, SU Tan, ZOU Bo. Synthesis and Fluorescence Properties of Novel Ce³⁺-doped Manganese Phosphite Open-framework Materials [J]. Chem. J. Chinese Universities, 2021, 42(9): 2725.
[6]	WU Yaqiang, LIU Siming, JIN Shunjin, YAN Yongqing, WANG Zhao, CHEN Lihua, SU Baolian. Synthesis of Zn-Doped NiCoP Catalyst with Porous Double-layer Nanoarray Structure and Its Electrocatalytic Properties for Hydrogen Evolution [J]. Chem. J. Chinese Universities, 2021, 42(8): 2483.
[7]	CHEN Mingsu, ZHANG Huiru, ZHANG Qi, LIU Jiaqin, WU Yucheng. First-principles Study on the Catalytic Effect of Co，P co-Doped MoS₂ in Lithium-sulfur Batteries [J]. Chem. J. Chinese Universities, 2021, 42(8): 2540.
[8]	MA Jun, ZHONG Yang, ZHANG Shanshan, HUANG Yijun, ZHANG Lipeng, LI Yaping, SUN Xiaoming, XIA Zhenhai. Design and Theoretical Calculation of Heteroatoms Doped Graphdiyne Towards Efficiently Catalyzing Oxygen Reduction and Evolution Reactions [J]. Chem. J. Chinese Universities, 2021, 42(2): 624.
[9]	CHEN Xiaoyu, YU Ranbo. Research Progress on Doping of Molybdenum Disulfide and Hydrogen Evolution Reaction [J]. Chem. J. Chinese Universities, 2021, 42(2): 475.
[10]	YAO Mingcai, YANG Qiang, MENG Jian, LIU Xiaojuan. Effect of Zn²⁺ Substituting Ga³⁺ on Structure and Photocatalytic Properties of Wurtzite β-CuGa_1-_xZn_xO₂with Unequal Doping [J]. Chem. J. Chinese Universities, 2021, 42(12): 3561.
[11]	HUANG Jialing,LIU Fengjiao,WANG Tingting,LIU Cuie,ZHENG Fengying,WANG Zhenhong,LI Shunxing. Nitrogen and Sulfur co-Doped Carbon Quantum Dots for Accurate Detection of pH in Gastric Juice^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1513.
[12]	ZHU Yuxin, OUYANG Jie, SONG Yanhua, TANG Sheng, CUI Yanjuan. Preparation of Boron and Iodine co-Doped Carbon Nitride and Its Performance in Photocatalytic Hydrogen Evolution from Water^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1645.
[13]	ZHAO Guoqing, YUAN Zhao, WANG Lian, GUO Zhuo. Preparation of Ni₂P/N, S co-Doped Reduced Graphene Oxide Composites and Their Electrocatalytic Properties for Hydrogen Evolution^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1575.
[14]	CHENG Shifu,HU Hao,CHEN Bihua,WU Haihong,GAO Guohua,HE Mingyuan. Preparation and Electrochemical Performance of Porous Carbons Prepared from Binary Ionic Liquids ^† [J]. Chem. J. Chinese Universities, 2020, 41(5): 1048.
[15]	WANG Xia, LIU Yanji, JIA Yongfeng, JI Lei, HU Quanli, DUAN Limei, LIU Jinghai. Preparative Chemistry of N-containing Porous Carbon Nanofibers for Capacity Improvement in Lithium-sulfur Battery ^† [J]. Chem. J. Chinese Universities, 2020, 41(4): 829.

Comparison of the Hydrolyzed Activity of β-Glucuronidases from Different Sources While Determining the Testosterone Level in Doping Control

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments