Determination of Characteristic Composition 10-Hydroxy-2-Decenoic Acid in Royal Jelly by High Performance Liquid Chromatography

Abstract

Abstract: Amethod for the quantitative determination of 10-hydroxy-2-decenoic acid (10-HDA) in royal jelly(RJ) and RJpreparation was established by high performance liquid chromatography (HPLC).The lo-HDAextracted with dichlo-romethane from royal jelly or royal jelly preparation was determined by HPLC. The system was recommended on a YWG-C₁₈ column (φ4.6×250 mm) using 35% ethanol in water (pH = 2.5) as the mobile phase; detection of ultraviolet absorption was performed at 212 nm, and at the range of 0.16 AUFS; internal standard, adipic acid.This method was successfully applied to the determination of 10-HDAcontents in raw royal jelly, ginseng royal jelly and ginseng pilose antler royal jelly. It is simpler, more rapid than and superior to gas chroma-tographic and thin-layer chromatographic methods.

Key words: HPLC, 10-Hydroxy-2-decenoic acid, Royal jelly, Ginseng royal jelly

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Zhang Xie, Fu Chunyan, Yang Renfei . Determination of Characteristic Composition 10-Hydroxy-2-Decenoic Acid in Royal Jelly by High Performance Liquid Chromatography[J]. Chem. J. Chinese Universities, 1989, 10(6): 609.

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Determination of Characteristic Composition 10-Hydroxy-2-Decenoic Acid in Royal Jelly by High Performance Liquid Chromatography

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