Studies on Hydrolysis and Polarographic Behavior of Amygdalin

Abstract

Abstract: Amygdalin is a non-active material.The polarographic study is based on the β-glucosidase-catalyzed hydrolysis of amygdalin to benzaldehyde.In a basic solution of injectio natrii chloridi the hy-drolysate of amygdalin exhibited a well defined reduction peak in a 2.5th differential polarogram, the peak potential was-1.340V (Vs.Ag/AgCl).The current was proportional to amygdalin concentration in the range of 5.9× 10^-7 to 1.4× 10^-4mol/L.With increasing pHin the basic solution, peak potential negatively shifted.Scanning rate, surfactant temperature and cyclic polarogram show that the reduction wave is a irreversible process controlled by difusion.Specific activity of β-glucosidase was first determined by polarography.The effect of various enzyme concentration on peak current was observed.

Key words: Amygdalin, Hydrolysis, Polarography

TrendMD:

Li Cang, Yuan Zhoubin . Studies on Hydrolysis and Polarographic Behavior of Amygdalin[J]. Chem. J. Chinese Universities, 1989, 10(11): 1150.

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Studies on Hydrolysis and Polarographic Behavior of Amygdalin

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