Chem. J. Chinese Universities ›› 2022, Vol. 43 ›› Issue (12): 20220546.doi: 10.7503/cjcu20220546
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Received:
2022-08-19
Online:
2022-12-10
Published:
2022-09-20
Contact:
HE Yao
E-mail:yaohe@suda.edu.cn
Supported by:
CLC Number:
TrendMD:
CHU Binbin, HE Yao. Silicon-based Nanoprobes for Imaging Detection and Therapy of Ocular Diseases[J]. Chem. J. Chinese Universities, 2022, 43(12): 20220546.
Fig.2 Silicon⁃based nanoprobes for imaging analysis and treatment of corneal NV[60](A) Schematic diagrams of the construction of corneal NV models; (B) slit-lamp images of corneal NV treated with alkaline solution(e.g., NaOH) after 1, 3, 5 and 7 d(scale bars: 5 mm); (C) confocal pictures of fluorescence imaging of corneal NV intravenously treated with pure SiNPs or the prepared SiNPs-RGD probes(scale bars: 25 μm); (D) corresponding slit-lamp images of corneal NV after the treatment with pure saline, SiNPs, RGD and the constructed SiNPs-RGD.Copyright 2018, the American Chemical Society.
Fig.3 Silicon⁃based nanoagents for rapid detection and effective treatment of bacterial keratitis(A) Schematic diagrams of the construction of the SiNPs-based NO delivery system; (B) schematic diagrams of the SiNPs-based NO delivery system for combating bacterial keratitis; (C) the slit-lamp images of bacteria-induced mouse corneas with different treatments[66]; (D) schematic diagrams for illustrating the preparation of SiNPs-Van nanoprobes; (E) confocal pictures of fluorescent imaging of S. aureus-infected or normal mouse corneas after the treatment of the constructed SiNPs-Van; (F) the slit-lamp images of S. aureus-induced mouse corneas after different treatments[67].(A—C) Copyright 2018, the American Chemical Society; (D—F) Copyright 2021, Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature.
Fig.4 SiNPs⁃based nanoplatform for the delivery of gene and treatment in RP diseases[77](A) Confocal images of HEK-293 cells after the treatment with the PRPF31-GFP plasmid-lading N-MSiNPs; (B) corresponding confocal images of HEK-293 cells treated with the PRPF31-GFP plasmid-lading N-MSiNPs; (C) fundus images; (D) fluorescence fundus; (E) detail of the injection site showing GFP fluorescent dots; (F) immunostaining of retinal sections. RPE represents retinal pigment epithelium, ONL represents outer nuclear layer, INL represents inner nuclear layer, GCL represents ganglion cell layer.Copyright 2022, Multidisciplinary Digital Publishing Institute.
Fig.5 Fluorescent SiNPs⁃based nanoagents for long⁃term and real⁃time imaging detection of retinal diseases in animal models[72](A) Schematic diagrams of fluorescent SiNPs-based probes; (B) schematic diagrams of the SiNPs probes for visual imaging detection of retina of rats in long term; (C) schematic diagrams of the prepared SiNPs probes for the imaging detection of retinal diseases of cynomolgus macaque; (D) fluorescence fundus images of the SiNPs- or FS-treated retinal vessels; (E) the bright field photograph of choroidal NV diseases of cynomolgus macaque after the treatment with the SiNPs; (F) corresponding fluorescence fundus images of choroidal NV of cynomolgus macaque with the treatment by the SiNPs.Copyright 2021, the Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature.
Fig.6 Long⁃term and real⁃time visualization of fluorescein⁃labeled MSNs moving from the ocular surface through the cornea into the anterior chamber[84](A) Schematic illustration of the main process of SNP@MSNs entering into the eye from the cornea. The NO donor could relax the Schlemm’s canal endothelial cells and trabecular meshwork cells and further induce the reduction of cell volume, resulting in lowering IOP; (B) fluorescence cross-section images of time-course distribution of the fluoresce-labeled MSNs in the mouse eyes. Scale bars: 100 μm. End: cornea endothelium; Epi: cornea epithelium; TM: trabecular meshwork; CB: ciliary body; SC: Schlemm’s canal.Copyright 2018, Wiley-VCH.
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