Anti-glioma Active Compounds and Their Nanomicelles Preparation of the Root of Actinidiaeriantha Benth†

doi:10.7503/cjcu20180507

Abstract

Abstract:

Actinidiaeriantha Benth was extracted by ethyl acetate, n-butyl alcohol and water, and three fractions were obtained. The in vitro cell experiments showed that ethyl acetate was the anti-glioma active fraction of the root of Actinidiaeriantha Benth. The high performance liquid chromatographic fingerprint confirmed that main compound of ethyl acetate fraction was 2α,3α,24-trihydroxyurs-12-en-28-oic acid, and it could significantly inhibit the glioma cell activity. The prepared 2α,3α,24-trihydroxyurs-12-en-28-oic acid loaded nanomicelles was with high loading efficiency of 64.7%, average diameter of 20 nm with polydispersity index(PDI) of 0.246 and ζ potential of -5.7 mV. Further cell experiment demonstrated the 2α,3α,24-trihydroxyurs-12-en-28-oic acid loaded nanomicelles through solvent evaporation with better inhibitory effects on the glioma cells than free one. Taken together, the ethyl acetate fractions from the root of Actinidiaeriantha Benth were confirmed with the anti-glioma activity, and 2α,3α,24-trihydroxyurs-12-en-28-oic acid was the main active compound. The prepared 2α,3α,24-trihydroxyurs-12-en-28-oic acid loaded nanomicelles was with good water dispersity and anti-glioma activity, providing solid experimental base for the application of the root of Actinidiaeriantha Benth in glioma therapy.

Key words: Root of Actinidiaeriantha Benth, 2α;, 3α;, 24-Trihydroxyurs-12-en-28-oic acid, Glioma, Active compound, Nanomicelle

TrendMD:

TANG Lin,JIANG Zhenqi,LI Juan,WU Aiguo. Anti-glioma Active Compounds and Their Nanomicelles Preparation of the Root of Actinidiaeriantha Benth^†[J]. Chem. J. Chinese Universities, 2019, 40(3): 468.

Figures/Tables 4

Fig.1 Cell viability of U87 MG cells after incubation with different extracts for 24 h n-Butanol extraction layer; ethyl acetate extraction layer; water extraction layer.

Fig.2 High performance liquid chromatogram of the standard sample(a), the n-butanol layer extract(b), the ethyl acetate layer extract(c) and the aqueous layer extract(d)

Fig.3 TEM image of 2α,3α,24-trihydroxy-12-en-28-oic acid-loaded nanomicelles

Fig.4 Cell inhibition effect of 2α,3α,24-trihydroxyurs-12-ene-28-olic acid() and its nanomicelles() on U87MG cell for 24 h

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