Abstract: Nineteen cases of glioma tissues(including 10 cases of astrocytic tumors and 9 cases of oligoastrocytic tumors) were detected using FT-mid-IR spectroscopy linked with attenuated total reflectance(ATR) accessory. In the astrocytic tumors cases, there were 4 cases of pure astrocytic tumors(WHOⅠ and Ⅱ), 2 cases of anaplastic astrocytic tumors(WHO Ⅲ) and 4 cases of glioblastoma(WHO Ⅳ). The FTIR spectra obtained from the brain tumor tissues were analyzed and compared. Significant differences were found in the peak position, band shape and intensity of infrared absorption spectra between the different grades of astrocytic tumors. The intensity of the band at ca. 1400 cm^-1 band is increased with the malignancy grade of astrocytic tumors. In addition, some spectral differences between astrocytic tumors and oligoastrocytic tumors were observed. In the spectra of oligoastrocytic tumors, the peak position of 1240 and 1300 cm^-1 shifted to low wavenumbers. And the band at ca. 1270 cm^-1 appeared in the spectra of oligoastrocytic tumors which was not observed in the astrocytic tumors. These results demonstrate that FT-mid-IR spectroscopy exhibits prospect to develop a novel, non-invasive and rapid method for the detection of brain tumors.

Key words: FT-mid-IR spectroscopy, Attenuate total reflection, Glioma