Preparation of Surface Enhanced Raman Scattering Substrates Based on SBA-15 Material and the Detection of Enrofloxacin in Chicken and Chicken Feed†

doi:10.7503/cjcu20170328

Abstract

Abstract:

A novel surface-enhanced raman scattering(SERS) substrate based on gold nanoparticles decorated amino group modified SBA-15(Au@NH₂-SBA-15) was developed. The synthetic conditions of the substrate were optimized including the time of amino modification and the volume of gold colloid using 4-mercaptopyridine(4-Mpy) served as a molecular probe, and the SERS performance was evaluated. The results showed that based on effective loading gold nanoparticles, the developed SERS substrate exhibited stronger SERS intensities and better stability with small signal reduction within five months comparing with traditional gold colloid substrate. The substrate was applied to detect enrofloxacin in chicken and chicken feed. The linear relationship between concentration of enrofloxacin and Raman intensity was good in the range of 0.1—1 mg/kg(R² equals 0.98 and 0.99 for the two samples), and the detection limit of enrofloxacin reached 100 μg/kg. The developed method with advantages of simpleness, rapidness, high sensitivity and good stability, contributed a favorable way to detect illegal additives of antibiotic with SERS.

Key words: Surface-enhanced Raman scattering(SERS) substrate, Enrofloxacin, Rapid detection, Mesoporous material

CLC Number:

O657.37

TrendMD:

SUN Lin, ZHANG Han, DU Yiping. Preparation of Surface Enhanced Raman Scattering Substrates Based on SBA-15 Material and the Detection of Enrofloxacin in Chicken and Chicken Feed^†[J]. Chem. J. Chinese Universities, 2018, 39(3): 455.

Figures/Tables 13

Fig.1 IR spectra(A) and XRD patterns(B) of SBA-15 before(a) and after(b) amino-modification

Fig.2 TEM images of SBA-15 before(A) and after(B) amino-modification

Fig.3 SEM images of Au@SBA-15(A) and Au@NH2-SBA-15(B)

Fig.4 SERS intensity line chart of 4-Mpy obtained on NH2-SBA-15 substrates with different amino-modification time

Fig.5 SERS intensity line chart of 4-Mpy obtained on substrates with different gold nanoparticles volumes

Fig.6 SERS spectra of 4-Mpy with the concentration of 10-7 mol/L collected from Au@NH2-SBA-15(a) and gold colloid(b)

Fig.7 SERS spectra of 4-Mpy with different concentrations collected from Au@NH2-SBA-15 substratec(Mpy)/(mol·L-1): a. 0; b. 10-10; c. 10-9; d. 10-8;e. 10-7; f. 10-6.

Fig.8 Average SERS intensities of corresponding bands of 1×10-7 mol/L 4-Mpy at 1093 cm-1(A) and 1580 cm-1(B) collected by three different batchesColumn number: a. 1; b. 2; c. 3.

Fig.9 SERS spectra collected randomly from substrate(A) and intensity distribution of the 1093 and 1580 cm-1 peaks in the 42 spectra(B)

Fig.10 SERS intensity versus stored time based on the measurement at 1092 and 1580 cm-1of 4-Mpy over 5 monthsEach data point represents the average value of 5 SERS spectra. Error bars show the standard deviations.

Fig.11 SERS spectra of enrofloxacin with the concentration of 10 mg/L(a) and 0 mg/L(b) collected from Au@NH2-SBA-15 SERS substrate

Fig.12 SERS spectra of enrofloxacin in chicken(A) and chicken food(B) with different concentrations and the intensity at 747 cm-1 of SERS spectra of enrofloxacin in chicken(C) and chicken food(D) in 0.1—10 mg/kgConcentration of enrofloxacin/(mg·kg-1): a. 100; b. 10; c. 1; d. 0.1; e. 0.01; f. 0.

Table 1 Comparison of different techniques for the detection capability of Enrofloxacin*

Technique	Lowest concentration/(μg·kg^-1)	Ref.
MI-SPE-HPCE	92.02	[18]
RPC-UV	600	[19]
HPLC-FLD	10	[20]
Silver sol film	43.13	[21]
Ag@GMA-EDMA	10	[16]
Au@NH₂-SBA-15	100	This work

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