Controlled Release of Multiple Hydrophilic and Hydrophobic Drugs and in vitro Cytotoxicity of Electrospun Poly(lactic-co-glycolic acid)/ZnO Nanofibers Encapsulated with Dual Drugs†

doi:10.7503/cjcu20140359

Abstract

Abstract:

A novel poly(lactic-co-glycolic acid)(PLGA)/ZnO electrospun composite fibers encapsulated with both hydrophilic drug(doxorubicin hydrochloride, DOX) and hydrophobic drug(camptothecin, CPT) were fabricated via electrospinning method. Primarily, the ZnO was decorat with F127 and then used to encapsulate DOX. Finally, the DOX-loaded ZnO(DOX@ZnO) and CPT were mixed with PLGA solution to fabricate electrospun hybrid nanofibers. The in vitro release results demonstrated the composite fibers decreased the burst release and increased the time of release, which showed a long-term and sustained release. The cell cytotoxicity test demonstated that the composite nanofiber with two drugs showed stronger antitumor efficacy against HepG-2 cells than the nanofiber with single drug. Thus, the composite nanofibers with two anticancer drugs could be a versatile drug delivery system as local implantable scaffolds for potential postsurgical cancer treatment.

Key words: Electrospinning, Nanofibers, ZnO, Drug delivery, Cytotoxicity, Poly(lactic-co-glycolic acid)

CLC Number:

O631

TrendMD:

ZENG Li, Hu Jun, WEI Junchao. Controlled Release of Multiple Hydrophilic and Hydrophobic Drugs and in vitro Cytotoxicity of Electrospun Poly(lactic-co-glycolic acid)/ZnO Nanofibers Encapsulated with Dual Drugs^†[J]. Chem. J. Chinese Universities, 2014, 35(8): 1788.

Figures/Tables 12

Fig.1 Schematic illustration of the process of fabrication of dual drug-loaded electrospun composite fiber

Fig.2 Synthesis of thiolated Pluronic F127

Fig.3 1H NMR spectra of F127(a) and HS-F127-SH(b)

Fig.4 FTIR spectra of ZnO NPs(a), HS-F127-SH(b) and FZnO NPs(c)

Fig.5 TGA curves of ZnO NPs(a) and FZnO NPs(b)

Fig.6 SEM images of ZnO NPs(A), FZnO NPs(B) and TEM image of FZnO NPs(C)

Fig.7 Size distribution of FZnO NPs

Fig.8 SEM images of neat PLGA fibers(A), FZnO/PLGA fibers(B), DOX@FZnO/PLGA fibers(C) and CPT/DOX@FZnO/PLGA fibers(D)

Fig.9 TEM images of neat PLGA fibers(A) and DOX@FZnO/PLGA fibers(B)

Fig.10 Cumulative DOX release from DOX@FZnO, DOX@FZnO/PLGA fibers and CPT/DOX@FZnO/PLGA composite fibers(A) and cumulative CPT release from CPT/DOX@FZnO/PLGA composite nanofibers(B)

Fig.11 In vitro cytotoxicity of the nanofiber mats to human liver carcinoma HepG-2 cells

Fig.12 CLSM images of HepG-2 cells co-cultured with blank(A), FZnO/PLGA fibers(B), DOX@FZnO/PLGA fibers(C) and CPT/DOX@FZnO/PLGA fibers(D)

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