高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (4): 549.

• 论文 • 上一篇    下一篇

N-羟甲基中卟啉诱导产生抗体的酶学性质研究

冯雁1, 高姝娟1, 高贵1, 刘锡煜1, 杨同书1, 刘玙2   

  1. 1. 吉林大学酶工程国家重点实验室, 长春, 130023;
    2. 中国人民解放军农牧大学研究所, 长春
  • 收稿日期:1994-05-12 修回日期:1994-11-30 出版日期:1995-04-24 发布日期:1995-04-24
  • 通讯作者: 冯雁,女,31岁,副教授.
  • 作者简介:冯雁,女,31岁,副教授.
  • 基金资助:

    国家教育委员会博士点基金

Studies on the Enzymological Properties of Antibody Induced by N-hydroxymethylmesohemin

FENG Yan1, GAO Shu-Juan1, GAO Gui1, LIU Xi-Yu1, YANG Tong-Shu1, LIU Zi2   

  1. 1. State Key Lab.of Enzyme Engineering, Jilin University, Changchun, 130023;
    2. Institute of Agriculture and Animal Science of PLA of China, Changchun
  • Received:1994-05-12 Revised:1994-11-30 Online:1995-04-24 Published:1995-04-24

PDF (PC)

被引次数

5

摘要： 以N-羟甲基中卟啉为半抗原,通过动物免疫、细胞融合等技术筛选得到的细胞株11D1,该细胞株产生的抗体具有结合铁中卟啉的能力,且表现出过氧化物酶活力。进一步研究表明,该抗体对半抗原及铁中卟啉的亲和力分别为:1.1×10-6mol/L和2.1×10-6mol/L;抗体-铁中卟啉复合物催化反应时,对底物过氧化氢的Km=13.0mmol/L,Kcat=86min-1,与自然催化速度相比,活力提高1.8×104(mol/L)-1.

关键词: N-羟甲基中卟啉, 抗体酶, 过氧化物酶

Abstract: N-hydroxymethylmesohemin was used as haptin to elicit monoclonal antibody 11D1through the immunization and cell fusion techniques.The antibody had the ability to combine with mesohemin and showed the activity of peroxidase.Further investigations demonstrate that the affinities of antibody for haptin and mesohemin were 1.1×10-6and 2.1×10-6mol/Lrespectively. When antibody-mesohemin complex catalyzed reaction, its Kmand Kcatfor H2O2were 13. 0 mmol/Land 86 min-1respectively. Compared with the noncat alyzed reaction, the activity of antibody-mesohemin complex elevated 1.8× 104(mol/L)-1.

Key words: N-hydroxymethylmesohemin, Abzyme, Peroxidase

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