高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (6): 1065.

• 研究论文 • 上一篇    下一篇

人源化甲状腺素脱碘酶单链抗体的结构模拟

林凤2, 章威3, 陈默1, 丁兰1, 刘兰英2, 徐筱杰3, 赵大庆1, 倪嘉缵1   

  1. 1. 中国科学院长春应用化学研究所稀土化学与物理开放实验室, 长春 130022;
    2. 吉林大学生命科学学院, 长春 130023;
    3. 北京大学化学与分子工程学院, 北京 100871
  • 收稿日期:2001-03-02 出版日期:2002-06-24 发布日期:2002-06-24
  • 通讯作者: 赵大庆(1963年出生),男,研究员,博士生导师,主要从事生物无机化学研究.E-mail:dqzhao@ns.ciac.jl.cn E-mail:dqzhao@ns.ciac.jl.cn
  • 基金资助:

    国家重大项目基金(批准号:KJ951-A1-504-02);中国科学院基础局基金(批准号:29701005);国家杰出青年科学基金(批准号:29625203)资助

Computer Simulation of Human Iodothyronine Deiodinase Single-chain Antibody

LIN Feng2, ZHANG Wei3, CHEN Mo1, DING Lan1, LIU Lan-Ying2, XU Xiao-Jie-ZHAOD a-Qing3, NI Jia-Zuan1   

  1. 1. Laboratory of Rare Earth Chemistry and Physics, Changchun Insitute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. College of Life Science, Jilin University, Chang chun 130023, China;
    3. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
  • Received:2001-03-02 Online:2002-06-24 Published:2002-06-24

PDF (PC)

被引次数

3

摘要： 以四碘甲状腺素原氨酸为半抗原,从人噬菌体展示半合成单链抗体库中筛选与半抗原有特异结合性的单链抗体E3,并测定了E3的一级结构,同时用计算机模拟了其空间结构.

关键词: 甲状腺素脱碘酶, 单链抗体, 计算机模拟

Abstract: Iodothyronine plays a major role in growth, basic metabolism and organ formation. It has an extremely limited source in the body. In this thesis, we designed iodothyronine(T4) as hapten. Then a single chain antibody displayed on phange was obtained from a human phage displaying a single chain antibody library. The specific genes of E3 was subcloned in P-5E vector. According to its amino acid sequences, we simulate its three-dimention structure by computer. It has never been reported in PDB.

Key words: Iodothyronine deiodinase, Single-chain antibody, Computer simulation

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