高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (1): 27.

• 论文 • 上一篇    下一篇

ss-DNA在纳米金上固载和杂化的电化学传感研究

缪谦, 金葆康, 林祥钦   

  1. 中国科学技术大学化学系, 合肥 230026
  • 收稿日期:1999-01-23 出版日期:2000-01-24 发布日期:2000-01-24
  • 通讯作者: 林祥钦(1946年出生),男,教授,博士生导师,主要从事电分析化学研究.
  • 基金资助:

    国家科技部基金(批准号:97KJ2904);中国科学院重点基金(批准号:9002KJ951-A1-507)资助

ss-DNA Immobilization and Hybridization on Nano-Gold/ 2-Aminoethane Thoil/Glassy Carbon Electrode

MIAO Qian, JIN Bao-Kang, LIN Xiang-Qin   

  1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
  • Received:1999-01-23 Online:2000-01-24 Published:2000-01-24

PDF (PC)

被引次数

188

摘要： 将2-氨乙基硫醇(AET)固载到玻碳电极(GCE)表面,进而化学吸附纳米金(NG),并在纳米金上固载ss-DNA得到ss-DNA/NG/AET/GCE,以Co(bpy)33+为电化学指示剂可以识别研究ss-DNA的杂化反应.结果表明,纳米金能使固载其上的ss-DNA发生部分变性而结合Co(bpy)33+,但用pH7.0的磷酸缓冲液浸泡可基本上避免这种变性,并明显提高杂化反应的识别能力.结合在ds-DNA/NG/AET/GCE上的Co(bpy)33+的峰电流与扫速的线性关系可保持到80mV/s.与电沉积法固载纳米金相比较,本电极更稳定和可靠.

关键词: DNA, 纳米金, 修饰电极, 杂化, 循环伏安

Abstract: The modification of glassy carbon electrode(GCE) with nano-gold(NG) by chemical adsorption of 2-aminoethane thoil(AET) was achieved for the first time. On this electrode 15-mer ss-DNA was immobilized, then ss-DNA/NG/AET/GCE was formed. By using Co(bpy)33+, hybridization events of the immobilized ss-DNA was well detected. It was found that the NG could possibly cause a part of the ss-DNA to denature and bind Co(bpy)33+. However, soaking in pH 7.0 phosphate buffer solution, denaturization can be avoided and recognition ability of hybridization was enhanced. Cyclic voltammetric behavior of Co(bpy)33+/2+ bound to the ds-DNA/NG/AET/GCE was studied. It was found that the peak current(ip) showed a linear dependence on the potential scan rate up to 80 mV/s. It was demonstrated that GCE modified with NG by this method had higher stability and reliability.

Key words: DNA, Nano-gold, Modified electrode, Hybridization, Cyclic voltammetry

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