高等学校化学学报

高等学校化学学报

• 研究简报 • 上一篇    下一篇

牛血清白蛋白对Cu2+-SCN--H2O2化学振荡系统影响作用的初步研究

孙涛1, 郭洪瑞2, 许环麟1, 周宝宽1   

    1. 天津医科大学化学教研室, 天津 300070;
    2. 南开大学化学学院, 天津 300071
  • 收稿日期:2006-10-09 修回日期:1900-01-01 出版日期:2007-05-10 发布日期:2007-05-10
  • 通讯作者: 周宝宽

Influence of BSA on Cu2+-SCN--H2O2 Chemical Oscillating System

SUN Tao1, GUO Hong-Rui2, XU Huan-Lin1, ZHOU Bao-Kuan1*   

    1. Department of Chemistry, Tianjin Medical University, Tianjin 300070, China;
    2. College of Chemistry, Nankai University, Tianjin 300071, China
  • Received:2006-10-09 Revised:1900-01-01 Online:2007-05-10 Published:2007-05-10
  • Contact: ZHOU Bao-Kuan

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摘要： 研究了牛血清白蛋白(BSA)对Cu2+-SCN--H2O2化学振荡系统的扰动作用, 观察并分析了BSA对该振荡体系的影响规律, 在此基础上提出了基于化学振荡系统的蛋白质定量检测方法.

关键词: 化学振荡, 牛血清白蛋白, 定量

Abstract: The influence of bovine serum albumin (BSA) on Cu2+-SCN--H2O2 chemical oscillating system was studied. The stable oscillating system contains 0.004 mol/L NaSCN, 0.066×10-3 mol/L CuSO4 and 0.111 mol/L H2O2, and it was injected during the peak of the third cycle of BSA. The electrode potential-time(E-t) curve was recorded and analyzed. The influence of the concentration of BSA on the chemical oscillating system's cycle prolonging(cycle-slope rate, RS) can be indicated in a quadratic term equation:
RS=1.94×10-3ρ(BSA)2+0.000595ρ(BSA)+0.059292
Protein may affect the chemical oscillation through midbody interference, binding with certain metallic ions, or alteration of solution's physical characteristics, but infraprotein may weaken the physical interference of the chemical oscillation by BSA. A linear relationship between infraprotein of BSA and oscillation cycle is derived with a coefficient correlation of R=0.996, and a linear range of 1—100 mg/L. Therefore, it is a new dynamic quantitative assay for the analysis of protein.

Key words: Chemical oscillation, Bovine serum albumin, Quantitative assay

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