高等学校化学学报

高等学校化学学报

• 研究简报 • 上一篇    下一篇

莲子草假隔链格孢毒素的分离纯化与结构鉴定

周遗品1, 向梅梅1, 姜子德2, 李华平2, 孙伟1, 林海琳1, 范怀忠2   

    1. 仲恺农业技术学院资源环境系, 广州 510225;
    2. 华南农业大学资源环境学院, 广州 510642
  • 收稿日期:2005-07-08 修回日期:1900-01-01 出版日期:2006-08-10 发布日期:2006-08-10
  • 通讯作者: 向梅梅

Separation, Purification and Structural Identification of the Phytotoxin from Nimbya Alternantherae

ZHOU Yi-Pin1, XIANG Mei-Mei1, JIANG Zi-De2, LI Hua-Ping2, SUN Wei1, LIN Hai-Lin1, FAN Huai-Zhong2   

    1. Department of Resources and Environment, Zhongkai University of Agriculture and Technology, Guangzhou 510225, China;
    2. College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China
  • Received:2005-07-08 Revised:1900-01-01 Online:2006-08-10 Published:2006-08-10
  • Contact: XIANG Mei-Mei

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被引次数

36

摘要： 从莲子草假隔链格孢(Nimbya alternantherae)中分离纯化了一种毒素物质, 并对其进行了结构鉴定, 为研究其致病机理及进一步的化学合成奠定了基础.

关键词: 莲子草假隔链格孢, 致病毒素, 分离纯化, 结构鉴定, 2-乙酰基-3,4-二羟基-5-甲氧基苯乙酸

Abstract: Nimbya alternantherae is a leaf spot fungus isolated from Alternanthera philoxeroides. It could produce phytotoxin to Alternanthera philoxeroides in several kinds of liquid media, especially in modified Fries. The filtrate of the pathogen was chromatographed on a DM-130 colophony column eluting with methanol, and evaporation of the solvent gave a brown mush. The crude product was then extracted with ethyl acetate. The toxin could be purified as white needles by re-crystallizing from benzene and acetone(volume ratio is 1:1) and silica gel chromatograph. The results of elemental analysis, MS, IR and NMR indicate that molecular weight of the toxin was 240, formula was C11H12O6, and its chemical name was 2-acetyl-3,4-dihydroxy-5-methoxyphenyl-acetic acid(Vulculic acid ).

Key words: Nimbya alternantherae, Phytotoxin, Separation and purification, Structural identification, 2-Acetyl-3,4-dihydroxy-5-methoxyphenyl-acetic acid

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