高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

荧光法研究三种黄酮小分子与溶菌酶的相互作用

杨冉1, 陈晓岚2, 李萍1, 屈凌波2   

    1. 中国药科大学现代中药教育部重点实验室, 南京 210038；
    2. 郑州大学化学系, 化学生物重点实验室, 郑州 450052
  • 收稿日期:2005-08-03 修回日期:1900-01-01 出版日期:2006-09-10 发布日期:2006-09-10
  • 通讯作者: 屈凌波

Interaction Between Lysozyme and Three Flavones by Fluorescence Spectroscopy

YANG Ran1, CHEN Xiao-Lan2, LI Ping1, QU Ling-Bo2   

    1. Key Laboratory of Modern Chinese Medicines, Ministry of Education, China Pharmaceutical University, Nanjing 210038, China;
    2. Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China
  • Received:2005-08-03 Revised:1900-01-01 Online:2006-09-10 Published:2006-09-10
  • Contact: QU Ling-Bo

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被引次数

53

摘要： 用荧光光谱法研究了芹菜素(Quercetin)、木犀草素(Luteocin)、槲皮素(Quercetin)与溶菌酶的相互作用, 对比了C3'—OH和C3—OH取代对黄酮与溶菌酶作用的影响. 结果表明, C3'—OH可大大增强黄酮与溶菌酶之间的作用, C3—OH的取代则导致作用力减弱. 根据3种黄酮的结构参数, 初步分析了C3'—OH和C3—OH取代对黄酮与蛋白相互作用的影响.

关键词: 荧光光谱法, 溶菌酶, 芹菜素, 木犀草素, 槲皮素, 能量转移, 结构参数

Abstract: The interaction between lysozyme and three flavones, including Apigenin, Luteocin and Quercetin, were studied by fluorescence spectroscopy. The influence of C3—OH and C3'—OH on the interaction of flavones-Lys were compared. The results showed that C3'—OH could enhance the binding affinity greatly; while C3—OH weakened the affinity largely. According to the structure parameters of assay flavones, the effect of hydrogen group on the flavones-protein were discussed, and the main reason resulting in the weaker binding affinity of Quercetin-Lys were explored. The results obtained from the work could supply beneficial reference for the further study of Flavone-Protein interactions.

Key words: Fluorescence spectroscopy, Lysozyme, Apigenin, Luteocin, Quercetin, Energy transfer, Structure parameter

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