高等学校化学学报

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半导体纳米粒子与金纳米粒子间荧光共振能量转移研究

张纪梅1, 代昭1, 郭宁1, 许世超1, 董全喜1, 孙波2   

    1. 天津工业大学材料化工学院, 天津 300160;
    2. 南开大学化学学院, 天津 300071
  • 收稿日期:2006-02-22 修回日期:1900-01-01 出版日期:2007-02-10 发布日期:2007-02-10
  • 通讯作者: 张纪梅

Fluorescence Resonance Energy Transfer Between CdTe Quantum Dot Donors and Au Nanoparticles Labeled DNA Acceptors

ZHANG Ji-Mei1, DAI Zhao1, GUO Ning1, XU Shi-Chao1, DONG Quan-Xi1, SUN Bo2   

    1. School of Material Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160;
    2. Chemistry School, Nankai University,Tianjin 300072,China
  • Received:2006-02-22 Revised:1900-01-01 Online:2007-02-10 Published:2007-02-10
  • Contact: ZHANG Ji-Mei

摘要: 采用水相法合成的CdTe半导体纳米粒子作为能量给体, 通过Schiff碱反应将单链DNA连接到表面. 采用柠檬酸钠还原氯金酸法制取的Au纳米粒子作为能量受体, 通过Au—S键将单链DNA连接到表面. 通过DNA链间的杂交, 构建了荧光共振能量转移体系(FRET). 测定了CdTe-DNA、 探针体系和探针体系+目标DNA的荧光强度. 结果表明, 探针体系的荧光强度最弱, 加入目标DNA后, 体系荧光增强, 表明该体系的构建是成功的.

关键词: 金纳米粒子, CdTe纳米粒子, 荧光共振能量转移, 荧光猝灭

Abstract: CdTe semiconductor nanoparticles which were synthesized in water phase were taken as the energy donors. CdTe nanoparticles were linked to 5'-NH2-DNA by Schiff's reaction. Au nanoparticles which were prepared by sodium citrate reduction of chloroauric acids were taken as the energy acceptors. Au nanoparticles were linked to 3'-SH—DNA by Au—S bond. The fluoresscence resonance energy transfer(FRET) probes are generated through hybridization of complementary DNA bound to the QDs and Au nanoparticles. The fluorescence spectra of CdTe-DNA, CdTe-DNA-Au and CdTe-DNA-Au+target DNA were measured . The data show that the fluorescence intensity of CdTe-DNA-Au conjugates was the weakest. When adding a complementary ssDNA to CdTe-DNA-Au conjugates, the fluorescence intensity of the system increased. This result shows that the configuration of FRET was successful.

Key words: Au nanoparticle, CdTe nanoparticle, Fluorescence resonance energy transfer(FRET), Fluorescence quenching

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