高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

[汤心颐先生诞辰100周年纪念专辑]基于三维枝状TiO2纳米阵列的三相酶催化反应界面及高效光电化学传感

王丹丹1,陈礼平2,封心建2   

  1. 1. 山西科技学院化学工程学院 2. 苏州大学材料与化学化工学部，仿生界面材料科学全国重点实验室
  • 收稿日期:2025-11-04 修回日期:2025-12-10 出版日期:2025-12-11 发布日期:2025-12-11
  • 通讯作者: 封心建 E-mail:xjfeng@suda.edu.cn
  • 基金资助:
    山西省高等学校科技创新项目(批准号:2024L452)，山西省基础研究计划资助项目(批准号:202403021222360)和国家重点研发计划项目(批准号: 2019YFA0709200)资助

Three-Phase Enzymatic Reaction Interface Based on 3D Branched TiO2 Nanoarrays for High Performance Photoelectrochemical Sensing

WANG Dandan1，CHEN Liping2，FENG Xinjian2   

  1. 1. School of Chemical Engineering, Shanxi Institute of Science and Technology 2. State Key Laboratory of Bioinspired Interfacial Materials Science, College of Chemistry, Chemical Engineering and Materials Science, Soochow University
  • Received:2025-11-04 Revised:2025-12-10 Online:2025-12-11 Published:2025-12-11
  • Contact: Xinjian Feng E-mail:xjfeng@suda.edu.cn
  • Supported by:
    Supported by the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (2024L452), the Fundamental Research Program of Shanxi Province (202403021222360) and the National Key Research and Development Program of China (No. 2019YFA0709200)

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摘要： 本文针对传统光电化学酶生物传感器溶解氧受限的核心问题，提出了一种基于三维枝状纳米阵列结构的“固-液-气三相反应界面”构建策略。该方法通过两步水热法在氟掺杂氧化锡导电玻璃上制备了具有三维枝状结构的TiO2纳米阵列，经过选择性疏水和亲水化处理与氧化酶修饰，成功构筑了稳定的三相界面。此设计实现了氧气可直接通过气相传输至催化位点，有效解决了传统固-液两相界面氧气供应不足的瓶颈。实验结果表明，该传感器的线性检测范围相较于传统结构提升了20倍，并表现出优异的稳定性（RSD < 2%）。本研究为开发高灵敏度、高稳定性的光电化学传感器提供了新的构筑策略，有望在慢性疾病的早期诊断中发挥重要作用。

关键词: 光电化学传感, 三维枝状TiO2纳米阵列, 三相界面, 氧化酶

Abstract: This study addresses the critical challenge of limited dissolved oxygen in traditional photoelectrochemical enzyme biosensors by proposing a construction strategy for a “solid-liquid-gas three-phase enzymatic reaction interface”, utilizing a three-dimensional (3D) dendritic nanostructure. The methodology involves the preparation of titanium dioxide (TiO2) nanowire arrays featuring a 3D dendritic structure on fluorine-doped tin oxide conductive glass through a two-step hydrothermal process. Following selective hydrophobic and hydrophilic treatments, along with enzyme modification, a stable three-phase interface was successfully established. This innovative design facilitates the direct transport of oxygen to the catalytic sites via the gas phase, effectively addressing the limitations associated with insufficient oxygen supply at the conventional solid-liquid two-phase interface. Experimental results demonstrate that the linear detection range of this sensor has been enhanced by a factor of 20 compared to traditional structures, while exhibiting excellent stability (relative standard deviation < 2%). This research introduces a novel construction strategy for the development of highly sensitive and stable photoelectrochemical sensors, which may significantly contribute to the early diagnosis of chronic diseases.

Key words: Photoelectrochemical biosensor, 3D branched TiO2 nanoarrays, Three-phase interface, Oxidase

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