高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (8): 1347-1353.doi: 10.7503/cjcu20170047

• 分析化学 • 上一篇    下一篇

虚拟分子印迹与表面增强拉曼散射联用技术用于孔雀石绿的超灵敏检测

马芳晨, 李欣, 任晓慧   

  1. 哈尔滨工业大学化工与化学学院, 城市水资源与水环境国家重点实验室, 新能源转换与储存关键材料技术工业和信息化部重点实验室, 哈尔滨 150090
  • 收稿日期:2017-01-20 修回日期:2017-06-30 出版日期:2017-08-10 发布日期:2017-06-30
  • 通讯作者: 李欣,男,博士,教授,博士生导师,主要从事分子印迹技术及表面增强拉曼散射光谱研究.E-mail:lixin@hit.edu.cn E-mail:lixin@hit.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:51579057,51379052)资助.

Ultrasensitive Detection of Malachite Green Using the Coupling Technique of Dummy Molecular Imprinting and Surface-enhanced Raman Scattering

MA Fangchen, LI Xin, REN Xiaohui   

  1. MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, State Key Lab of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150090, China
  • Received:2017-01-20 Revised:2017-06-30 Online:2017-08-10 Published:2017-06-30
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.51579057, 51379052).

摘要:

采用抗坏血酸还原法制备Ag球粒子,然后用3-(甲基丙烯酰氧)丙基三甲氧基硅烷使其表面硅烷化,最后用虚拟模板分子松香酸代替模板分子孔雀石绿与功能单体甲基丙烯酰胺反应合成虚拟印迹聚合物.结果表明,生成的"核-壳"式复合基底比Ag的表面增强拉曼散射(SERS)增强效果显著,其对孔雀石绿的最低检测浓度达到10-11 mol/L.该方法实现了背景噪音的消除,提高了分析结果的准确性,为有机染料的超灵敏检测提供了参考.

关键词: 虚拟分子印迹聚合物, 表面增强拉曼散射, 孔雀石绿, 银粒子

Abstract:

The coupling technique of molecular imprinting with surface enhanced Raman scattering is regarded as a promising analysis method due to its properties of high sensitivity and selectivity. However, the traditional molecular imprinting technique is very difficult to thoroughly remove template molecules. So, the technique will result in strong background interference impacting the test results when it is combined with the surface-enhanced Raman scattering spectroscopy. In this study, the Ag particles were first synthesized adopting the method of ascorbic acid reduction. Then, the as-prepared Ag particles were silanized using 3-methacryloxypropyltrimethoxysilane. Finally, the dummy molecularly imprinted polymers were synthesized using abietic acid replacing malachite green as the template molecule to react with methacrylamide functional monomer. The results show that the resulting "core-shell" composite substrate has a more remarkable SERS enhancement than Ag substrate. And its detection limit for malachite green can down to 10-11 mol/L. This method can eliminate the background interference and improve the accuracy of the analysis results, which provide a new method for the ultrasensitive detection of organic dyes.

Key words: Dummy molecularly imprinted polymer, Surface-enhanced Raman scattering, Malachite green, Ag particles

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