生物兼容蛋白水凝胶微图案的光刻平版印刷

doi:10.7503/cjcu20150733

高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (4): 715.doi: 10.7503/cjcu20150733

生物兼容蛋白水凝胶微图案的光刻平版印刷

郑博元¹, 纪锋颖², 侯智善¹, 孙思明¹, 孙允陆¹(), 杨蕊竹³()

1. 吉林大学电子科学与工程学院, 集成光电子学国家重点联合实验室, 长春 130012
2. 青岛市疾病预防控制中心, 青岛 266033
3. 表面物理与化学重点实验室, 绵阳 621907

收稿日期:2015-09-21 出版日期:2016-04-10 发布日期:2016-03-22
基金资助:
国防预研究基金(批准号: 9140c661404030c66278)和国家自然科学基金(批准号: 51373064)资助

Preparation of Biocompatible Protein-hydrogel-based Micro-patterns via UV Lithography^†

ZHENG Boyuan¹, JI Fengying², HOU Zhishan¹, SUN Siming¹, SUN Yunlu^1,^*(), YANG Ruizhu^3,^*()

1. State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, China
2. Qingdao Municipal Center for Disease Control and Prevention, Qingdao 266033, China
3. Science and Technology on Surface Physics and Chemistry Laboratory, Mianyang 621907, China

Received:2015-09-21 Online:2016-04-10 Published:2016-03-22
Contact: SUN Yunlu,YANG Ruizhu E-mail:sunyunlu825@163.com;jonas_bamboo@126.com
Supported by:
† Supported by the National Defense Pre-Research Foundation of China(No.9140c661404030c66278) and the National Natural Science Foundation of China(No.51373064)

摘要/Abstract

摘要：

通过简易的紫外光刻平版印刷技术, 对无化学修饰的生物兼容性良好的天然牛白蛋白的水相光刻胶进行微图案化加工, 获得了可用作微光栅器件的蛋白质微条纹结构, 表征了蛋白质凝胶微条纹图案的宏观性状. 结果表明, 其折射散射彩虹色明显, 单束激光经过后的衍射条纹对称度高, 可获得11 级衍射条纹. 通过改变参数实现了条纹的可控褶皱. 将图案化的蛋白水凝胶膜用于肝癌细胞的培养, 实现了细胞图案化排布.

关键词: 紫外光刻, 蛋白质水凝胶, 微光栅, 生物兼容性, 微褶皱

Abstract:

Through facile UV lithography, without chemical modification, biocompatible natural protein-hydrogel-based film and micro-patterns were prepared. The micro-patterns can be used as micro-grating optical devices. Then rainbow feature was observed and diffraction fringe for a 638 nm laser beam was obtained under white light, even with a diffraction level up to 11. Further more, micro-wrinkles could be controlled by changing related parameters. Finally, HepG-2 cells were cultured on the protein micro-patterns and cell micro-patterns were obtained.

Key words: UV Lithography, Protein hydrogel, Micro-grating, Bio-compatibility, Micro-wrinkle

中图分类号:

O644.11

TrendMD:

郑博元, 纪锋颖, 侯智善, 孙思明, 孙允陆, 杨蕊竹. 生物兼容蛋白水凝胶微图案的光刻平版印刷. 高等学校化学学报, 2016, 37(4): 715.

ZHENG Boyuan, JI Fengying, HOU Zhishan, SUN Siming, SUN Yunlu, YANG Ruizhu. Preparation of Biocompatible Protein-hydrogel-based Micro-patterns via UV Lithography^†. Chem. J. Chinese Universities, 2016, 37(4): 715.

图/表 8

Fig.1 Spectrum of the UV light source(A) and diagram of UV-lithographing protein gratings(B)

Fig.2 Optical microscopic images of protein gratings^The insets are the grating masks for UV-lithographing. Period/μm: (A) 55; (B) 25; (C) 15; (D) 10.

Fig.3 Optical characterization of the micro-gratings ^(A)—(D) Rainbow images shown by the protein-based micro-patterns under white light source; (E) Protein-based micro-pattern as micro-grating optical device(diffraction fringes of a 638 nm laser beam). The inset shows the diffractive optical testing system.

Scheme 1 Several possible reactions of histidine-involved protein photo-crosslinking

Fig.4 AFM images of micro-gratings^Period/μm: (A) 10; (B) 15; (C) 25; (D) 55.

Fig.5 Smooth protein hydrogel micro-stripe structure via optimized UV-lithographing^(A) AFM image of the smooth protein hydrogel micro-structure; (B) AFM 3D profile of the structure in (A); (C) the cross-section profile at position marked by the line in (A).

Fig.6 Cell micropatterning via the protein microstructures^(A), (B) Optical microscopic images of HepG-2 cells cultured on the protein micro-patterns; (C) SEM image of patterned cells on protein microstructures; (D) cross-section SEM image of patterned cells on protein microstructures.

Fig.7 3D AFM profile of a cell guided along protein-hydrogel microstructure

参考文献 38

[1]	Llobera1 A., Juvert J., González-Fernández1 A., Ibarlucea1 B., Carregal-Romero1 E., Büttgenbach S., Fernández-Sánchez C., Light: Science & Applications, 2015, 4(4), e271
[2]	Li N., Tittl A., Yue S., Giessen H., Song C., Ding B. Q., Liu N., Light: Science & Applications, 2014, 3, e226
[3]	Deming T. J., Advanced Materials, 1997, 9(4), 299—311
[4]	Felix J. M., Gatenholm P., Journal of Applied Polymer Science, 1991, 42(3), 609—620
[5]	Hu T., Kaplan D. L., Omenetto F. G., Advanced Materials, 2012, 24(21), 2824—2837
[6]	Oh H. H., Ko Y. G., Lu H. X., Kawazoe N., Chen G. P., Advanced Materials, 2012, 24(31), 4311—4316
[7]	Sun Y.L., Sun S. M., Wang P., Dong W. F., Zhang L., Xu B. B., Chen Q. D., Tong L. M., Sun H. B.,Small, 2015, 2869—2876
[8]	Bar-Noam A. S., Farah N., Shoham S., Light: Science & Applications, 2016, 5(1), e16007
[9]	Hong S., Lee M. Y., Jackson A. O., Lee L. P., Light: Science & Applications, 2015, 4, e267
[10]	Liang K., Ricco R., Doherty C. M., Styles M. J., Bell S., Kirby N., Mudie S., Haylock D., Hill A. J., Doonan C. J., Falcaro P., Nature Communications, 2015, 6(8240), 1—8
[11]	Pincella F., Isozaki K., Miki K., Light: Science & Applications, 2014, 3(1), e133
[12]	Urness A. C., Moore E. D., Kamysiak K. K., Cole M. C., Mcleod R. R., Light: Science & Applications, 2013, 2(3), e56
[13]	Calafiore G., Koshelev A., Dhuey S., Goltsov A., Sasorov P., Babin S., Yankov V., Cabrini S., Peroz C., Light: Science & Applications, 2014, 3, e203
[14]	Zhang W., Zappe H., Seifert A., Light: Science & Applications, 2014, 3(2), e145
[15]	Kawata S., Sun H. B., Nature, 2001, 412(6848), 697—698
[16]	Sugioka K., Cheng Y., Light: Science & Applications, 2014, 3(4), e149
[17]	Wu D., Xu J., Niu L. G., Wu S. Z., Midorikawa K., Sugioka K., Light: Science & Applications, 2015, 4(4), e228
[18]	Wang C., Zhu F., Ren L., Li A. W., Chen C., Yang R., Guo J. C., Xue Y., Zhang X. Y., Zhu C. C., Chen Q. D., Yu Y. S., Chem. Res. Chinese Universities, 2013, 29(6), 1199—1202
[19]	Xiong W., Zhou Y. S., He X. N., Gao Y., Mahjouri-Samani M., Jiang L., Baldacchini T., Lu Y.F., Light: Science & Applications, 2012, 1, e6
[20]	Kumar K., Lee K. K., Li J., Nogami J., Kherani N. P., Herman P. R., Light: Science & Applications, 2014, 3(1), e157
[21]	Vannahme C., Dufva M., Kristensen A., Light: Science & Applications, 2015, 4(4), e269
[22]	Gou M.L., Qu X., Zhu W., Xiang M. L., Yang J., Zhang K., Wei Y. Q., Chen S. C., Nature Communications, 2014, 5(4774), 1—9
[23]	Xia H., Wang J., Tian Y., Chen Q. D., Du X. B., Zhang Y. L., He Y., Sun H. B., Advanced Materials, 2010, 22, 3204—3207
[24]	Yang J., Luo F. F., Kao T. S., Li X., Ho G. W., Teng J. H., Luo X. G., Hong M. H., Light: Science & Applications, 2014, 3(7), e185
[25]	Zhang Y. L., Xia H., Kim E., Sun H. B., Soft Matter, 2012, 8, 11217—11231
[26]	Wu D., Wu S. Z., Chen Q. D., Zhang Y. L., Yao J., Yao X., Niu L. G., Wang J. N., Jiang L., Sun H. B., Advanced Materials, 2011, 23, 545—549
[27]	Liebetraut P., Petsch S., Liebeskind J., Zappe H., Light: Science & Applications, 2013, 2(9), e98
[28]	Wu D., Chen Q. D., Niu L. G., Wang J. N., Wang J., Wang R., Xia H., Sun H. B., Lab. Chip., 2009, 9, 2391—2394
[29]	Zhang Y. L., Guo L., Wei S., He Y. Y., Xia H., Chen Q. D., Sun H. B., Xiao F. S., Nano Today, 2010, 5, 15—20
[30]	Guo L., Jiang H. B., Shao R. Q., Zhang Y. L., Xie S. Y., Wang J. N., Li X. B., Jiang F., Chen Q. D., Zhang T., Sun H. B., Carbon, 2012, 50(4), 1667—1673
[31]	Sun Y. L., Dong W. F., Yang R. Z., Meng X., Zhang L., Chen Q. D., Sun H. B., Angew. Chem. Int. Edit., 2012, 51, 1558—1562
[32]	Sun Y. L., Dong W. F., Niu L. G., Jiang T., Liu D. X., Zhang L., Wang Y. S., Chen Q. D., Kim D. P., Sun H. B., Light: Science & Applications, 2014, 3, e129
[33]	Kurland N. E., Dey T., Kundu S. C., Yadavalli V. K., Advanced Materials, 2013, 25, 6207—6212
[34]	Shen H. R., Spikes J. D., Smith C. J., Kopecek J., Journal of Photochemistry and Photobiology A: Chemistry, 2000, 130(2000), 1—6
[35]	Fancy D. A., Kodadek T., Proceedings of the National Academy of Sciences of the United States of America, 1999, 96, 6020—6024
[36]	Kochevar I. E., Lynch M. C., Zhuang S. G., Lambert C. R., Photochem. Photobiol., 2000, 72, 548—554
[37]	Chan E. P., Crosby A. J., Advanced Materials, 2006, 18, 3238—3242
[38]	Wang J. M., Wang C., Wang M. C., Jiang L., Chem. J. Chinese Universities, 2011, 32(8), 1807—1811
	(王景明, 王春, 王明超, 江雷, 高等学校化学学报, 2011, 32(8), 1807—1811)

生物兼容蛋白水凝胶微图案的光刻平版印刷

Preparation of Biocompatible Protein-hydrogel-based Micro-patterns via UV Lithography^†

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生物兼容蛋白水凝胶微图案的光刻平版印刷

Preparation of Biocompatible Protein-hydrogel-based Micro-patterns via UV Lithography†

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Preparation of Biocompatible Protein-hydrogel-based Micro-patterns via UV Lithography^†