高等学校化学学报

高等学校化学学报 ›› 1989, Vol. 10 ›› Issue (3): 229.

• 研究论文 • 上一篇    下一篇

过氧化物酶模拟酶催化荧光反应的研究(Ⅰ)--MnTPPS4-HVA-H2O2体系

慈云祥, 王芳   

  1. 北京大学化学系
  • 收稿日期:1988-01-27 出版日期:1989-03-24 发布日期:1989-03-24
  • 通讯作者: 慈云祥
  • 基金资助:

    国家自然科学基金

Studies on Fluorescence Reaction Catalyzed by Mimetic Enzyme of Peroxidase--System of MnTPPS4-HVA-H2O2

Ci Yunxiang, Wang Fang   

  1. Department of Chemistry, Peking University, Beijing
  • Received:1988-01-27 Online:1989-03-24 Published:1989-03-24

PDF (PC)

摘要： 首次研究了meso-四(4-磺基苯)卟啉和锰的络合物(MnTPPS4)作为过氧化物酶模拟酶对高香草酸(HVA)和H2O2荧光反应的催化作用,拟订了用模拟酶测定痕量H2O2和葡萄糖的荧光测定方法,并对模拟酶和天然酶的性能进行了比较。用MnTPPS4测定H2O2和葡萄糖的灵敏度分别可以达到8.5×10-8mol/L的H2O2和0.5μgmL-1的葡萄糖。

关键词: 过氧化物酶模拟酶, 荧光法测定过氧化氢, 葡萄糖

Abstract: A fluorescence reaction of homovanillic acid with hydrogen peroxide catalyzed by mimetic enzyme of peroxidase (manganese tetrakis(sulfophenyl) porphyrin) was investigated. Ahigh sensitive fluorometric method for the determination of hydrogen peroxide and glucose has been developed. The sensitivities of the determination of hydrogen peroxide and glucose are 8.5×10-8mol/Lfor hydrogen peroxide and 0.5μg·mL-1for glucose, respectively. The recommended method was applied to determine hydrogen peroxide in snow and glucose in human serum and satisfactory results were obtained.

Key words: Mimetic enzyme of peroxidase, Determination of hydrogen peroxide by fluorescence method, Glucose

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