高等学校化学学报

高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (2): 281.

• 论文 • 上一篇    

模拟过氧化物酶催化性能的研究

孙淑声, 王春海   

  1. 北京大学化学系, 100871
  • 收稿日期:1989-06-22 出版日期:1991-02-24 发布日期:1991-02-24
  • 通讯作者: 孙淑声
  • 基金资助:

    国家自然科学基金

Studies on Catalytic Characteristic of Mimetic Peroxidase

Sun Shu-sheng, Wang Chun-hai   

  1. Department of Chemistry, Peking University, Beijing, 100871
  • Received:1989-06-22 Online:1991-02-24 Published:1991-02-24

PDF (PC)

被引次数

2

摘要： 过氧化物酶(POD)能催化H2O2氧化一系列氢供体(DH2)转化成氧化型供体(D)。氢供体可以是邻联二茴香胺、4-氨基安替比林和苯酚及某些染料隐色体等。这类反应不仅可提供检测H2O2的灵敏方法,还可与产生H2O2的其它酶反应系统联用,测定葡萄糖、半乳糖、氨基酸、尿酸及胆醇等生物物质。

关键词: 过氧化物酶, 模拟过氧化物酶, 人血清申葡萄糖测定

Abstract: Amimetic peroxidase, chelate of manganese with tetrakis (3-N-methylpyridyl)-porphyrin was synthesized, and its catalytic characteristic of chromogenic reaction of hydrogen peroxide with 4-aminoantipyrine and N,N-diethylaniline was investigated. Asensitive spectrophotometric method for the determination of trace hydrogen peroxide is developed. The glucose in human serum was determined by the proposed reaction combining with glucose oxidase system with satisfactory results.

Key words: Peroxidase, Mimetic peroxidase, Determination of glucose in human serum

TrendMD: