高等学校化学学报

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巯基乙酸自组装膜DNA电化学传感器对转基因NOS的定量检测

孙伟, 尚智美, 杨茂霞, 焦奎   

  1. 青岛科技大学化学与分子工程学院, 青岛 266042
  • 收稿日期:2006-01-09 修回日期:1900-01-01 出版日期:2006-10-10 发布日期:2006-10-10
  • 通讯作者: 孙伟

Mercaptoacetic Acid Self-assembled DNA Electrochemical Biosensor for Detection of NOS Gene Sequence

SUN Wei, SHANG Zhi-Mei, YANG Mao-Xia, JIAO Kui   

  1. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
  • Received:2006-01-09 Revised:1900-01-01 Online:2006-10-10 Published:2006-10-10
  • Contact: SUN Wei

摘要: 以转基因植物中常用的根癌农杆菌终止子(NOS)为检测对象, 将巯基乙酸自组装于金电极表面形成巯基乙酸自组装单分子膜, 再利用乙基-(3-二甲基氨丙基)碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺(NHS)的活化作用将NOS探针ssDNA序列固定于金电极表面形成NOS电化学生物传感器, 以亚甲基蓝(MB)为杂交指示剂, 对NOS靶基因相关序列进行了定量检测.

关键词: 电化学基因传感器, 巯基乙酸, NOS基因, 亚甲基蓝, 自组装膜电极

Abstract: A NOS gene electrochemical biosensor was prepared by covalently mmobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS). By using methylene blue as the electrochemical indicator, the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8—1.0×10-4 mol/L with the linear regression equation as ΔIpc=1.10lgc+8.25(n=8, γ=0.998 5) and the detection limit as 3.60×10-8 mol/L(3σ).

Key words: Electrochemical DNA biosensor, Mercaptoacetic acid, NOS gene, Methylene blue, Self-assembled film electrode

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