疏水疏油纳米银修饰不锈钢材料的制备及生物相容性

doi:10.7503/cjcu20150296

摘要/Abstract

摘要：

采用恒电位电化学沉积法在316L不锈钢表面沉积银纳米修饰层, 并置于全氟硅烷溶液中进行疏水修饰, 通过控制沉积时间和修饰过程, 制备了一系列具有不同界面亲疏水特性的修饰材料. 采用扫描电子显微镜(SEM)、能量散射X 射线光谱(EDX)仪及接触角测量仪等对材料性能进行了表征; 利用四甲基偶氮唑盐(MTT )法和流式细胞仪等检验了材料的血液相容性; 并将修饰后的316L不锈钢植入实验动物体内, 检验其组织相容性. 实验结果表明, 该纳米银全氟硅烷修饰的316L不锈钢材料比316L裸不锈钢具有更好的血液及组织相容性, 有望成为一种理想的血管支架应用材料.

关键词: 电化学沉积, 界面修饰, 生物相容性

Abstract:

Ag nanoparticles films were prepared by the potentiostatic electrodeposition strategy onto the surface of 316L stainless steel. The thin films were treated with an avantin solution of 1,1,2,2-perfluorooctyl triethoxysilane(25%, volume fraction) for 20 h to make surface hydrophobic. By controlling the deposition time and modification process, a series of modified materials with different hydrophilic and hydrophobic properties was prepared. Characterizations by means of scanning electron microscopy(SEM), energy dispersive X-ray spectroscopy(EDX) as well as contact angle measurements were performed. Then, MTT technique and flow cytometry examinations were employed for further investigations of the biocompatibility of this film towards human blood. Such modified 316L stainless steel was subsequently implanted into the experimental animals to study the biocompatibility towards tissue. It is verified that compared with the bare 316L stainless steel, the Ag nanoparticles films modified substrate demonstrated preferable blood and tissue compatibility. Such kind of modified material with improved hydrophobic superiority may be a promising candidate for further practical application as an ideal vascular scaffold stuff.

Key words: Electrochemical deposition, Interfacial modification, Biocompatibility

中图分类号:

O646

TrendMD:

王智慧, 薛歆, 邢玥, 李淑梅, 刘斌, 韩冬雪, 牛利. 疏水疏油纳米银修饰不锈钢材料的制备及生物相容性. 高等学校化学学报, 2015, 36(8): 1542.

WANG Zhihui, XUE Xin, XING Yue, LI Shumei, LIU Bin, HAN Dongxue, NIU Li. Preparation and Biocompatibility of Ag Nanoparticles Modified Stainless Steel^†. Chem. J. Chinese Universities, 2015, 36(8): 1542.

图/表 5

Fig.1 SEM images of 316L stainless steels with various time of electrochemically deposited Ag coats(A)100 s; (B) 500 s; (C) 800 s; (D) 1000 s; (E) 1500 s; (F) EDX pattern of sample (E).

Fig.2 Contact angles of water at electrochemical deposited Ag(a) and electrochemically deposited Ag/PFOTES modified 316L stainless steels(b) with various timeInset is photograph of water drop at electrochemically deposited Ag(4000 s)/PFOTES modified 316L stainless steels.

Table 1 OD values of different group at different time

Experiment material	24 h	72 h	120 h	168 h
Control group	0.173±0.0239	0.413±0.0265	0.767±0.0519	0.834±0.0467
Bare stainless steel	0.168±0.0203	0.376±0.0323	0.757±0.0535	0.809±0.0578
800 s group	0.179±0.0285	0.430±0.0266	0.734±0.0587	0.817±0.0532
1600 s group	0.189±0.0246	0.387±0.0286	0.729±0.0423	0.868±0.0534
2000 s group	0.175±0.0308	0.381±0.0405	0.721±0.0525	0.780±0.0480
4000 s group	0.180±0.0269	0.398±0.0298	0.720±0.0632	0.785±0.0626

Table 2 Ratio of stage in cell cycle of different groups by FCM

Experiment material	G0/G1 stage(%)	S stage(%)	M stage(%)
Control group	65.3±4.07	20.3±3.05	13.5±1.64
Bare stainless steel	66.8±3.71	17.6±2.62	12.6±1.63
800 s group	67.2±3.39	17.1±3.08	13.5±2.07
1600 s group	65.6±3.51	17.5±1.68	13.4+1.58
2000 s group	68.7±3.60	17.1±3.74	13.0±2.75
4000 s group	68.6±4.56	16.8±2.63	13.5±2.34

Fig.3 IVUS images of pre and after stent implantation(A)Pre-stent implantation of bare stainless steel group; (B) after stent implantation of bare metal group; (C) 30 d after stent implantation of bare stainless steel group; (D) 30 d after stent implantation of 800 s group; (E) 30 d after stent implantation of 1600 s group; (F) 30 d after stent implantation of 2000 s group; (G) 30 d after stent implantation of 4000 s group.

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