应用代谢轮廓区分不同致病性副溶血性弧菌

doi:10.3969/j.issn.0251-0790.2012.08.010

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (08): 1686.doi: 10.3969/j.issn.0251-0790.2012.08.010

应用代谢轮廓区分不同致病性副溶血性弧菌

赵强, 孙晓红, 卢瑛, 潘迎捷, 赵勇

上海海洋大学食品学院, 上海水产品加工及贮藏工程技术研究中心, 上海 201306

收稿日期:2011-10-08 出版日期:2012-08-10 发布日期:2012-08-10
通讯作者: 赵勇, 男, 博士, 副教授, 主要从事食品安全学研究. E-mail: yzhao@shou.edu.cn E-mail:zhaoyong1@163.com
基金资助:
上海市科技兴农重点攻关项目(批准号: 沪农科攻字2006第10-5号); 上海市科学技术委员会部分地方院校能力建设项目(批准号: 11310501100)和上海市科学技术委员会工程中心建设项目(批准号: 11DZ2280300)资助.

Application of Metabolic Profiling for Distinguishing Different Pathogenic Vibrio Parahaemolyticus

ZHAO Qiang, SUN Xiao-Hong, LU Ying, PAN Ying-Jie, ZHAO Yong

College of Food Science & Technology, Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai Ocean University, Shanghai 201306, China

Received:2011-10-08 Online:2012-08-10 Published:2012-08-10

摘要/Abstract

摘要： 基于tdh, trh和tlh 3个基因区分了不同的致病性副溶血性弧菌. 采用液相色谱(LC)和气相色谱-质谱联用(GC-MS)技术获得不同的致病性副溶血性弧菌的代谢轮廓, 并将其用于区分不同的致病性副溶血性弧菌; 同时以肠杆菌基因间重复共有序列聚合酶链反应技术(ERIC-PCR)及DNA重复序列PCR技术(REP-PCR)为对照, 采用NTsys2.10e软件计算所得结果的相似系数, 并对气相色谱-质谱联用结果进行解析. 结果表明, 根据所得代谢轮廓可以很好地区分不同的致病性副溶血性弧菌; 对气相色谱-质谱联用分析结果解析发现了不同致病性菌株的潜在生物标志物: tdh⁺, trh^-, tlh⁺菌株3种, tdh^-, trh⁺, tlh⁺菌株2种, tdh^-, trh^-, tlh⁺菌株 3种.

关键词: 副溶血性弧菌, 致病性, 代谢轮廓, 区分, 潜在生物标志物

Abstract: Distinguish of different pathogenic vibrio parahaemolyticus are normally based on tdh, trh and tlhgenes. In this research, the metabolic profiles were obtained by liquid chromatography(LC) and gas chromatography/mass spectrometry(GC-MS), and they were used for distinguishing different pathogenic vibrio parahaemolyticus. The results were compared with E. coli gene sequence between the repeat PCR(ERIC-PCR) and DNA repeats-PCR(REP-PCR). The similarity was calculated by NTsys2.10e software. The results showed that different pathogenic Vibrio parahaemolyticus could be distinguished by LC and GC/MS. Potential biomarkers of different pathogenic strains were found by GC-MS. Strain of tdh⁺, trh^-, tlh⁺ have 3 kinds of potential biomarkers, strain of tdh^-, trh⁺, tlh⁺ have 2 kinds of potential biomarkers, strain of tdh^-, trh^-, tlh⁺ have 3 kinds of potential biomarkers.

Key words: Vibrio parahaemolyticus, Pathogenic, Metabolic profiling, Distinguish, Potential biomarker

中图分类号:

O657

TrendMD:

赵强, 孙晓红, 卢瑛, 潘迎捷, 赵勇. 应用代谢轮廓区分不同致病性副溶血性弧菌. 高等学校化学学报, 2012, 33(08): 1686.

ZHAO Qiang, SUN Xiao-Hong, LU Ying, PAN Ying-Jie, ZHAO Yong. Application of Metabolic Profiling for Distinguishing Different Pathogenic Vibrio Parahaemolyticus. Chem. J. Chinese Universities, 2012, 33(08): 1686.

参考文献

[1] LI Xiao-Chun(李晓春). China Preventive Medicine(中国预防医学杂志)[J], 2004, 5(5): 370-373 [2] Su Y. C., Liu C. C.. Food Microbio.[J], 2007, 24: 549-558 [3] Vongxay K.. Sci. Food. Agric.[J], 2006, 86: 1871-1877 [4] LIU Hui-Hui(刘慧慧), XUE Chao-Bo(薛超波). Chinese Journal of Preventive Veterinary Medicine(中国预防兽医学报)[J], 2010, 32(1): 23-26 [5] Lee C., Chen L. H., Liu M. L., Su Y. C.. Appl. Environ. Microbiol.[J], 1992, 58: 3419-3422 [6] Levin R. E.. Food Biotech.[J], 2006, 20: 92-128 [7] DOU Yong(窦勇), HU Pei-Hong(胡佩红). Modern Food Science and Technology(现代食品科技)[J], 2008, 24(6): 598-602 [8] DU Yu-Ping(杜玉萍), CHEN Qing(陈清), KE Xue-Mei(柯雪梅). South China Journal of Preventive Medicine(华南预防医学)[J], 2007, 1: 19- 21 [9] Okuda J., Nishibuchi M.. Mol. Microbiol.[J], 1998, 30(3): 499-511 [10] Oltvai Z. N., Barabasi A. L.. Science[J], 2002, 298(5594): 763-764 [11] Barabasi A. L., Oltvai Z. N.. Nat. Rev. Genet.[J], 2004, 5(2): 101-115 [12] Boxrud D.. Current Opinion in Biotechnology[J], 2010, 21: 1-5 [13] Buchholz A., Takors R., Wandrey C.. Anal. Biochem.[J], 2001, 295: 129-137 [14] Boroujerdi A. F., Vizcaino M. I., Meyers A., Pollock E. C., Huynh S. L., Schock T. B., Morris P. J., Bearden D. W.. Environ. Sci. Technol.[J], 2009, 43(20): 7658-7664 [15] Bourne R., Himmelreich U., Sharma A., Mountford C., Sorrell T.. J. Clin. Microbiol.[J], 2001, 39(8): 2916-2923 [16] de Bruijn F. J.. Applied and Environmental Microbiology[J], 1992, 58(7): 2180-2187 [17] Versalovic J., Koeuth T., Lupski J. R.. Nucl. Acids Res.[J], 1991, 19: 6823-6831 [18] MEI Hui(梅辉), DAI Jun(戴军), LIU Wen-Wei(刘文卫), LING Xia(凌霞), ZHU Peng-Fei(朱鹏飞), ZHAO Zhi-Jun(赵志军). Chin. J. Anal. Chem.(分析化学)[J], 2011, 39(4): 534-539 [19] LIANG Xiao-Ping(梁晓萍), ZHANG Zheng(张政), HU Ping(胡坪), ZHONG Hong-Fu(钟宏福), GE Zhao-Hui(葛朝晖), ZOU Ting-Ting(邹婷婷), LIANG Qiong-Lin(梁琼麟), WANG Yi-Ming(王义明), LUO Guo-An(罗国安). Chem. J. Chinese Universities(高等学校化学学报)[J], 2011, 32(1): 38-43 [20] WANG Wei-Hua(王伟华), LIU Chun-Hong(柳春红), SUN Yuan-Ming(孙远明), LIN Feng(林峰), ZHANG Ming-Ming(张明明), WANG Zhao-Bin(王兆斌), ZHANG Min-Yi(张敏仪). Chem. J. Chinese Universities(高等学校化学学报)[J], 2011, 32(10): 2280-2285 [21] ZHU Chao(朱超), HUANG Min(黄敏), LIANG Qiong-Lin(梁琼麟), WANG Yi-Ming(王义明), HU Ping(胡坪), LI Ping(李平), ZHANG Hao-Jun(张浩军), LU Xiao-Guang(路晓光), LUO Guo-An(罗国安). Chem. J. Chinese Universities(高等学校化学学报)[J], 2011, 32(7): 1512-1518 [22] Kim Y. M., Farrah S., Baney R. H.. Electronic Journal of Biotechnology[J], 2006, 9(2): 176-180 [23] Gutiérrez-García A. G., Contreras C. M., Remedios Mendoza-López M. R., García-Barradas O., Cruz-Sánchez J. S.. Physiology & Behavior[J], 2007, 91: 166-172 [24] Wang Z. S., Sindreu C. B., Li V., Nudelman A., Chan G. C. K., Storm D. R.. The Journal of Neuroscience[J], 2006, 26(28): 7375-7379 [25] McCarley K. D., Bunge A. L.. Journal of Pharmaceutical Sciences[J], 2001, 90(11): 1699-1719 [26] Bushara K. O., Goldstein S. R., Grimes G. J. Jr., Burstein A. H., Hallett M.. Neurology[J], 2004, 62(1): 122-124

应用代谢轮廓区分不同致病性副溶血性弧菌

Application of Metabolic Profiling for Distinguishing Different Pathogenic Vibrio Parahaemolyticus

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价

[1]	赵幻希, 王秋颖, 孙秀丽, 李雪, 苗瑞, 吴冬雪, 刘淑莹, 修洋. HPLC-MS结合多元统计分析区分人参产地及筛选皂苷类标志物[J]. 高等学校化学学报, 2019, 40(2): 246.
[2]	王姜, 李倩, 顾海巍, 郭晓暾, 杨水平, 王志豪. 表面解吸常压化学电离质谱法直接分析牙齿微区表面[J]. 高等学校化学学报, 2015, 36(6): 1067.
[3]	黄如丹, 乔正平, 李白涛, 费宝丽, 王恩波. 区分α-,β-[SiW₉M₃(H₂O)₃O₃₉]^7-(M=Al³⁺,Ga³⁺)Keggin结构三取代杂多阴离子几何异构体新方法[J]. 高等学校化学学报, 1999, 20(3): 353.
[4]	范崇正, 铃木稔, 井上嘉, 安部忠广. 青铜生锈过程中铜元素的扩散─CuCl的作用[J]. 高等学校化学学报, 1994, 15(12): 1737.