高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (9): 2071.

• 综合评述 • 上一篇    下一篇

脂筏微区超分子聚集体结构的稳定性

孙润广1, 张静2, 郝长春1, 陈莹莹1, 杨谦1   

  1. 1.  陕西师范大学物理学与信息技术学院,   生物物理与生物医学工程研究室,
    2.   食品工程与营养科学学院, 西安 710062
  • 收稿日期:2011-04-06 修回日期:2011-06-19 出版日期:2011-09-10 发布日期:2011-08-11
  • 通讯作者: 孙润广 E-mail:sunrunguang@snnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:  20772077)和教育部科学技术研究重点项目(批准号:  104167)资助.

Stabilities of Supramolecular Complex Structures in Lipid Rafts Microdomains

SUN Run-Guang1*, ZHANG Jing2, HAO Chang-Chun1, CHEN Ying-Ying1, YANG Qian1   

  1. 1.  Laboratory of Biophysics and Biomedical Engineering, College of Physics and Information Technology,
    2.  College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China
  • Received:2011-04-06 Revised:2011-06-19 Online:2011-09-10 Published:2011-08-11
  • Contact: SUN Run-Guang E-mail:sunrunguang@snnu.edu.cn
  • Supported by:

    国家自然科学基金(批准号:  20772077)和教育部科学技术研究重点项目(批准号:  104167)资助.

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被引次数

9

摘要： 生物膜中脂筏微区结构的动态特征与稳定性决定着生物膜的功能。通过从动物细胞提取脂筏，实验不但观测到质膜微囊烧瓶状凹陷结构，而且还观测到大量的球状和椭球状结构.通过模拟脂筏微区结构，重点对二元体系和三元体系的超分子聚集体结构的多形性进行了研究和探索。研究发现随着表面压力的增加，鞘磷脂和胆固醇双层膜出现了紧密聚集不规则的微区结构，在 SM/Chol/DOPC双层膜中，SM/Chol形成的微区结构漂浮在液态DOPC小颗粒上部。当 DOPE加入到SM/Chol中，三种成份形成不稳定的双层膜结构.Ceramide促进了SM/Chol结构发生重排，微区形状从原来的不规则向着紧密聚集的圆形结构演变；混合单层膜的分子面积与表面吉布斯自由能决定了分子间的相互作用, 当过量分子面积与过量吉布斯自由能为负值时，分子间相互作用表现为吸引力, 出现凝聚现象; 为正值时，分子间相互作用表现为排斥力, 促使单层膜出现相分离现象. 过量吉布斯自由能值越小, 单层膜的热稳定性越高.通过动物细胞提取脂筏与体外模拟脂筏相结合的方法，从超分子水平阐述了脂筏微区结构与功能的生物学意义，为生物膜的研究提供了理论依据和实验支持。

关键词: 脂筏;  , 微区结构;  , 超分子聚集体;  , 动态特征;  , 稳定性

Abstract: The dynamic and stability characteristics of lipid raft in the biomembrane have great effect on many biological processes. Extracted lipid rafts from animal cells, not only flask-shaped caveolae but also spherical and ellipsoidal morphology have been observed. Simulated the lipid raft in vitro and focused on the binary and ternary system to study the polymorphisms of membrane structure. The results showed that SM/Chol bilayer appeared tightly clustered irregular microdomains with the increase in surface pressure. For the SM/Chol/DOPC bilayer system, the microdomains formed by SM/Chol floated on the fluid small DOPC area. When DOPE added to the SM/Chol, the bilayers structure became unstable. Ceramide induced SM/Chol bilayer rearrangement, from original irregular structure to closely aggregated round structure. In the mixed monolayers, the molecular area and surface Gibbs free energy determines the molecular interactions. When the excess molecular and excess Gibbs energy is negative deviations from ideality, the interaction between molecules is attractive and the monolayer is more aggregated. Positive deviation from linearity indicates repelling interaction and the monolayer forms phase separation structure. The less the Gibbs energy value, more stable the monolayer is. Combined extraction from animal cells and simulated in vitro methods, study the biological significance of lipid raft between structure and function from super-molecular level. This project conduction can provide theoretical evidence and experimental support for studying biomembrane.

Key words: Lipid raft;  , Microdomain;  , Supramolecular aggregate;  , Dynamic characteristic;  , Stability

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